Abstract: Provided herein are materials and methods for producing diploid, fertile, uniform, and vigorous hybrid potato. Also provided are methods of using advanced breeding methods, such as genome design, to generate potato inbred lines with high homozygosity which enables the exploitation of heterosis in this tuber crop and transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one.

Abstract: Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Ss11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

Abstract: Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Ss11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.

Abstract: Provided herein are materials and methods for producing diploid, fertile, uniform, and vigorous hybrid potato. Also provided are methods of using advanced breeding methods, such as genome design, to generate potato inbred lines with high homozygosity which enables the exploitation of heterosis in this tuber crop and transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one.

Abstract: Disclosed is a method for breeding self-compatible potatoes, including the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method; and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Ss11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent, and selecting a self-incompatible material as the male parent, and then obtaining a self-compatible F1 generation by hybridization. The invention overcomes the self-incompatibility of diploid potatoes, and does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.