Patents by Inventor Dieter C. Gruenert

Dieter C. Gruenert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for EST-specific full length cDNA cloning
    Patent number: 6265165
    Abstract: A method for cloning of single EST-specific full length cDNA, and/or multiplex-EST-specific full-length cDNA cloning. The method involves cDNA synthesis from highly enriched, homogeneously purified mRNAs and includes hybrid selection for purification of specific mRNAs from total RNA by employing antisense oligonucleotide primers based on expressed sequence tag (EST) sequences.
    Type: Grant
    Filed: January 29, 1999
    Date of Patent: July 24, 2001
    Assignee: The Regents of the University of California
    Inventors: Zhidong Xu, Dieter C. Gruenert
  • Method and assay for detection of the expression of allele-specific mutations by allele-specific in situ reverse transcriptase polymerase chain reaction
    Patent number: 5804383
    Abstract: A method and an allele-specific in situ reverse transcriptase polymerase chain reaction (RT-PCR) amplification assay for detection and differentiation between expression of unmutated wild-type DNA sequences and between endogenous mutated DNA sequences in vitro or in vivo. The method is useful for verification, diagnostic assessment and monitoring of therapeutic small fragment homologous replacement gene therapy, for diagnostic assessment and monitoring of cDNA-based gene therapies, for analysis of gene expression of specific alleles during fetal development and for diagnostic assessment of the expression of alleles involved in cancer mutations.
    Type: Grant
    Filed: October 8, 1996
    Date of Patent: September 8, 1998
    Assignee: The Regents of the University of California
    Inventors: Dieter C. Gruenert, Austin F. Dohrman