Patents by Inventor Dieter Klaubert

Dieter Klaubert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DETECTION OF HYDROGEN PEROXIDE
    Publication number: 20140329257
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Application
    Filed: July 21, 2014
    Publication date: November 6, 2014
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • COMPOSITIONS AND METHODS FOR CAPTURE OF CELLULAR TARGETS OF BIOACTIVE AGENTS
    Publication number: 20140287427
    Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
    Type: Application
    Filed: March 11, 2014
    Publication date: September 25, 2014
    Applicant: Promega Corporation
    Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Tetsuo Uyeda, Keith Wood
  • Detection of hydrogen peroxide
    Patent number: 8785652
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: July 22, 2014
    Assignee: Promega Corporation
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • COMPOSITIONS AND METHODS FOR CAPTURE OF CELLULAR TARGETS OF BIOACTIVE AGENTS
    Publication number: 20140199712
    Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
    Type: Application
    Filed: December 12, 2013
    Publication date: July 17, 2014
    Applicant: Promega Corporation
    Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood
  • Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
    Patent number: 8779221
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: May 22, 2012
    Date of Patent: July 15, 2014
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance Encell, Tonny Johnson, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • Luminescence-based methods and probes for measuring cytochrome P450 activity
    Patent number: 8765969
    Abstract: The invention provides compounds, compositions, methods, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferins or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.
    Type: Grant
    Filed: July 3, 2008
    Date of Patent: July 1, 2014
    Assignee: Promega Corporation
    Inventors: James J. Cali, Dieter Klaubert, William Daily, Samuel K. Ho, Susan Frackman, Erika Hawkins, Keith V. Wood
  • LUMINOGENIC AND FLUOROGENIC COMPOUNDS AND METHODS TO DETECT MOLECULES OR CONDITIONS
    Publication number: 20140154716
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: June 10, 2013
    Publication date: June 5, 2014
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John W. Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Polynucleotide encoding a mutant dehalogenase to allow tethering to functional groups and substrates
    Patent number: 8742086
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: June 3, 2014
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • COMPOUNDS AND METHODS FOR ASSAYING REDOX STATE OF METABOLICALLY ACTIVE CELLS AND METHODS FOR MEASURING NAD(P)/NAD(P)H
    Publication number: 20140093894
    Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.
    Type: Application
    Filed: September 20, 2013
    Publication date: April 3, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: Helene A. BENINK, James J. CALI, Sarah DUELLMAN, Dieter KLAUBERT, Donna LEIPPE, Martha O'BRIEN, John SHULTZ, Jolanta VIDUGIRIENE, Wenhui ZHOU, Mary SOBOL
  • COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR
    Publication number: 20130337539
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: May 15, 2013
    Publication date: December 19, 2013
    Applicant: PROMEGA CORPORATION
    Inventors: Aldis Darzins, Lance Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
    Patent number: 8476450
    Abstract: A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.
    Type: Grant
    Filed: July 3, 2008
    Date of Patent: July 2, 2013
    Assignee: Promega Corporation
    Inventors: James J. Cali, William Daily, Erika Hawkins, Dieter Klaubert, Jianquan Liu, Poncho Meisenheimer, Michael Scurria, John Shultz, James Unch, Michael P. Valley, Keith V. Wood, Wenhui Zhou
  • Covalent tethering of functional groups to proteins and substrates therefor
    Patent number: 8466269
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: June 18, 2013
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • COMPOUNDS AND METHODS FOR ASSAYING REDOX STATE OF METABOLICALLY ACTIVE CELLS AND METHODS FOR MEASURING NAD(P)/NAD(P)H
    Publication number: 20130130289
    Abstract: The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement.
    Type: Application
    Filed: August 31, 2012
    Publication date: May 23, 2013
    Applicant: PROMEGA CORPORATION
    Inventors: Helene A. BENINK, James J. CALI, Sarah DUELLMAN, Dieter KLAUBERT, Donna LEIPPE, Martha O'BRIEN, John SHULTZ, Jolanta VIDUGIRIENE, Wenhui ZHOU, Mary SOBOL
  • DETECTION OF HYDROGEN PEROXIDE
    Publication number: 20130045497
    Abstract: The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same.
    Type: Application
    Filed: August 16, 2012
    Publication date: February 21, 2013
    Applicant: Promega Corporation
    Inventors: Dieter Klaubert, John Shultz, James Unch, Michael P. Valley, Hui Wang, Wenhui Zhou
  • METHOD OF IMMOBILIZING A PROTEIN OR MOLECULE VIA A MUTANT DEHALOGENASE THAT IS BOUND TO AN IMMOBILIZED DEHALOGENASE SUBSTRATE AND LINKED DIRECTLY OR INDIRECTLY TO THE PROTEIN OR MOLECULE
    Publication number: 20120330001
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: May 22, 2012
    Publication date: December 27, 2012
    Applicant: PROMEGA CORPORATION
    Inventors: Aldis Darzins, Lance Encell, Tonny Johnson, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • Reactive cyanine compounds
    Patent number: 8309059
    Abstract: The invention provides compounds and compositions of Formulas I-VII, and methods of using the compounds. The compounds can be used to prepare dye conjugates that are uniformly and substantially more fluorescent on proteins, nucleic acids or other biopolymers, than conjugates labeled with structurally similar known carbocyanine dyes. In addition to having more intense fluorescence emission than structurally similar dyes at virtually identical wavelengths, and decreased artifacts in their absorption spectra upon conjugation to biopolymers, the compounds can have greater photostability and/or higher absorbance (extinction coefficients) at the wavelength(s) of peak absorbance than such structurally similar dyes.
    Type: Grant
    Filed: August 31, 2010
    Date of Patent: November 13, 2012
    Assignee: Promega Corporation
    Inventors: Cesear Corona, Dieter Klaubert, Mark McDougall
  • COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A RADIONUCLIDE AND METHODS OF USE
    Publication number: 20120258470
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: April 18, 2012
    Publication date: October 11, 2012
    Applicant: PROMEGA CORPORATION
    Inventors: Keith V. Wood, Dieter Klaubert, Georgyi V. Los, Robert F. Bulleit, Mark McDougall, Chad Zimprich
  • COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A CONTRAST AGENT AND METHODS OF USE
    Publication number: 20120252048
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: April 18, 2012
    Publication date: October 4, 2012
    Applicant: PROMEGA CORPORATION
    Inventors: Keith V. Wood, Dieter Klaubert, Georgyi V. Los, Robert F. Bulleit, Mark McDougall, Chad Zimprich
  • Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use
    Patent number: 8257939
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: December 21, 2010
    Date of Patent: September 4, 2012
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Georgyi V. Los, Robert F. Bulleit, Dieter Klaubert, Mark McDougall, Chad Zimprich
  • COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR
    Publication number: 20120220013
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: March 30, 2012
    Publication date: August 30, 2012
    Applicant: PROMEGA CORPORATION
    Inventors: Aldis Darzins, Lance Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich