Abstract: The present invention provides a test carrier for the determination of an analyte from whole blood with the help of reagents contained in the test carrier, said test carrier having a blood application side to which blood is applied, an evaluation side on which, as a result of the reaction of the reagents with the analyte, an optically detectable change takes place, and an erythrocyte separation means between the blood application side and the evaluation side, wherein the erythrocyte separation means includes an integral composite having a first zone, which contains a polymeric film former, kieselguhr and a pigment, and thereupon, with formation of a transition region, a second zone containing a polymeric film-former, formed by liquid coating the first zone facing the blood application side and the second zone facing the evaluation side of the integral composite. The present invention also provides a process for the production of this test carrier and a method for analyzing blood samples using it.