Patents by Inventor Dieter Trau
Dieter Trau has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10927406Abstract: A process for identifying one or more target analytes present in a sample includes contacting a microarray with the sample. The microarray has at least two microbead subpopulations with randomly distributed spatial positions, each of the subpopulations comprising microbeads having a known active agent capable of binding with a respective target analyte. After contacting the microarray with the sample, the spatial positions of the microbeads which have undergone a change that is indicative of a respective one of the active agents being bound to a corresponding one of the target analytes are determined. At least one of the microbead subpopulations which has undergone the change is identified by comparing the determined spatial positions to recorded spatial positions of the microbeads of the microbead subpopulations stored in an encoding/decoding data table. The target analytes are identified based on the known active agents belonging to the at least one identified microbead subpopulation.Type: GrantFiled: June 15, 2016Date of Patent: February 23, 2021Assignee: NATIONAL UNIVERSITY OF SINGAPOREInventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
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Patent number: 10203324Abstract: A method for determining a presence or absence of one or more target analytes in a sample includes contacting the sample with an array of particles comprising at least first and second particle subsets disposed therein with a known particle number ratio with respect to each other. The first particle subset has at least one binding site configured to bind with a first target analyte and the second particle subset has at least one binding site configured to bind with a second, different target analyte. Changes are detected in a detectable signal emitted by the particles after contacting the sample with the array. A number of the particles that emit the change in the detectable signal are counted and this number is compared to the known particle number ratio of the subsets so as to determine the presence or absence of the one or more of the target analytes.Type: GrantFiled: September 30, 2016Date of Patent: February 12, 2019Assignee: NATIONAL UNIVERSITY OF SINGAPOREInventor: Dieter Trau
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Publication number: 20180011120Abstract: A system and methods for consumer use of a microarray product are provided. A method includes obtaining a microarray product and contacting the microarray product with a sample and performing bioassays. The method further includes obtaining a decoding information data package from a manufacturer of the microarray product for correlating bioassay signals from the bioassays with respective analytes, wherein the microarray product is obtained at a first time different and discrete from a second time that the decoding information data package is obtained.Type: ApplicationFiled: June 2, 2013Publication date: January 11, 2018Inventors: Dieter Trau, Andreas Schmidt
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Publication number: 20170038371Abstract: A method for determining a presence or absence of one or more target analytes in a sample includes contacting the sample with an array of particles comprising at least first and second particle subsets disposed therein with a known particle number ratio with respect to each other. The first particle subset has at least one binding site configured to bind with a first target analyte and the second particle subset has at least one binding site configured to bind with a second, different target analyte. Changes are detected in a detectable signal emitted by the particles after contacting the sample with the array. A number of the particles that emit the change in the detectable signal are counted and this number is compared to the known particle number ratio of the subsets so as to determine the presence or absence of the one or more of the target analytes.Type: ApplicationFiled: September 30, 2016Publication date: February 9, 2017Inventor: Dieter TRAU
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Patent number: 9486770Abstract: A method of encoding a microarray includes depositing a first batch of particles on a substrate. The first batch of particles comprises a mixture of at least two sub-batches of the particles. Each of the sub-batches is capable of binding to a different, specific target analyte. Information necessary for decoding the microarray is provided prior to or during the depositing and includes a unique particle number ratio of the sub-batches. If more than one batch of particles is deposited, an image can be taken of the first batch prior to depositing subsequent batches to provide information about the position of the particles in the first batch of particles. These depositing and imaging steps can be sequentially repeated for the subsequent batches. Such a microarray having multiple batches can be decoded using the information about the position of the particles and the known particle number ratios of the sub-batches.Type: GrantFiled: July 19, 2013Date of Patent: November 8, 2016Assignee: NATIONAL UNIVERSITY OF SINGAPOREInventor: Dieter Trau
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Publication number: 20160291006Abstract: A process for identifying one or more target analytes present in a sample includes contacting a microarray with the sample. The microarray has at least two microbead subpopulations with randomly distributed spatial positions, each of the subpopulations comprising microbeads having a known active agent capable of binding with a respective target analyte. After contacting the microarray with the sample, the spatial positions of the microbeads which have undergone a change that is indicative of a respective one of the active agents being bound to a corresponding one of the target analytes are determined. At least one of the microbead subpopulations which has undergone the change is identified by comparing the determined spatial positions to recorded spatial positions of the microbeads of the microbead subpopulations stored in an encoding/decoding data table. The target analytes are identified based on the known active agents belonging to the at least one identified microbead subpopulation.Type: ApplicationFiled: June 15, 2016Publication date: October 6, 2016Inventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson NG
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Patent number: 9395360Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.Type: GrantFiled: October 21, 2013Date of Patent: July 19, 2016Assignee: NATIONAL UNIVERSITY OF SINGAPOREInventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
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Publication number: 20150290333Abstract: This disclosure relates to a fully or partially biodegradable carrier for the delivery of biologically active agents which are associated, either directly or indirectly, with the carrier via a biodegradable linking agent and the use of the carrier in the delivery of bioactive molecules for therapy and imaging, in particular the delivery of agents to mitochondria.Type: ApplicationFiled: October 30, 2013Publication date: October 15, 2015Applicant: National University of SingaporeInventors: Michael Raghunath, Sebastian Beyer, Rafi Rashid, Anna Maria Blocki, Dieter Trau, Thorsten Wohland
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Publication number: 20150182932Abstract: A microarray for detecting a presence of one or more target analytes in a sample includes an array of particles having one or more binding sites thereon for binding with the one or more target analytes present in the sample. The array of particles has at least two particle subsets. Each of the subsets has at least one binding site to one or more of the target analytes that are unique to the respective subset. A number of particles of each of the subsets is known and the known number of particles of each of the subsets is useable to generate a ratio value of particle subsets such that a presence of two or more of the target analytes in the sample is thereby detectable.Type: ApplicationFiled: July 19, 2013Publication date: July 2, 2015Inventor: Dieter Trau
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Publication number: 20150148262Abstract: A bead construct includes a bead, a fluorophore molecule, first and second linker molecules and a ligand molecule. The fluorophore molecule comprises at least two subunits connected to each other by at least one internal linker that provides conformational freedom to the at least two subunits. An optical signal emitted by the fluorophore molecule changes when reducing the conformational freedom of the at least two subunits to each other. The first linker molecule is connected to the bead and to one of the subunits of the flurophore molecule. The second linker molecule is connected to another one of the subunits of the fluorophore molecule. The ligand molecule is connected to the second linker molecule.Type: ApplicationFiled: May 22, 2013Publication date: May 28, 2015Applicant: NATIONAL UNIVERSITY OF SINGAPOREInventors: Dieter Trau, Martin James Lear, Christopher Ochs
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Patent number: 8927465Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.Type: GrantFiled: August 3, 2007Date of Patent: January 6, 2015Assignee: National University University of SingaporeInventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
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Publication number: 20140357504Abstract: A system and methods for consumer use of a microarray product are provided. A method includes obtaining a microarray product and contacting the microarray product with a sample and performing bioassays. The method further includes obtaining a decoding information data package from a manufacturer of the microarray product for correlating bioassay signals from the bioassays with respective analytes, wherein the microarray product is obtained at a first time different and discrete from a second time that the decoding information data package is obtained.Type: ApplicationFiled: June 2, 2013Publication date: December 4, 2014Inventors: Dieter Trau, Andreas Schmidt
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Publication number: 20140045721Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.Type: ApplicationFiled: October 21, 2013Publication date: February 13, 2014Applicant: National University of SingaporeInventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson NG
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Publication number: 20100075865Abstract: A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray.Type: ApplicationFiled: August 3, 2007Publication date: March 25, 2010Applicant: NATIONAL UNIVERSITY OF SINGAPOREInventors: Dieter Trau, Wen-Tso Liu, Kian Kok Johnson Ng
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Patent number: 7045146Abstract: Process for encapsulation of an uncharged crystalline solid particle include treating the crystalline solid particle material with amphiphilic substances and subsequently coating the material with a layer of charged polyelectrolyte or coating the material with a multilayer comprising alternating layers of oppositely charged polyelectrolytes.Type: GrantFiled: January 12, 2001Date of Patent: May 16, 2006Assignee: Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.Inventors: Frank Caruso, Dieter Trau, Helmuth Möhwald, Reinhard Renneberg
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Publication number: 20050202429Abstract: The present invention refers to microcapsules with high permeability for low molecular weight molecules and no permeability for high molecular weight molecules encapsulating a nucleic acid amplification reaction mixture, in particular, a PCR reaction mixture and providing a compartment to perform a nucleic acid amplification, in particular, a PCR reaction and to applications of such capsules to perform parallel PCR, screening, construction of DNA libraries and sequencing.Type: ApplicationFiled: March 20, 2003Publication date: September 15, 2005Applicant: Innovativebio.BizInventors: Dieter Trau, Reinhard Renneberg, Mak Wing Cheung
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Patent number: 6833192Abstract: The invention refers to a new process for preparing coated crystals by coating crystal template particles with alternating layers of oppositely charged polyelectrolytes and/or nanoparticles.Type: GrantFiled: March 25, 2002Date of Patent: December 21, 2004Assignee: Max-Planck Gesellschaft Zur Forderrung der Wissenschaften E.V.Inventors: Frank Caruso, Dieter Trau, Helmuth Möhwald, Reinhard Renneberg
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Patent number: 6686208Abstract: The invention relates to devices and methods for carrying out quantitative fluorescence immunoassays using evanescent field excitation. Light from at least one light source is directed onto the boundary between two media which have differing refractive indices. The light source emits practically monochromatic light with a wavelength suitable for exciting a marking substance. The light is directed onto a boundary surface disposed between an optically transparent base plate, the refractive index of which is greater than that of the material above the boundary surface, and a receiving region for the sample. The receiving region is covered with a covering plate on the side disposed opposite the base plate, there being arranged between the base plate and covering plate at least one functional layer. A detector for detecting the fluorescent light is disposed on the same side of the base plate as the light source.Type: GrantFiled: August 6, 2002Date of Patent: February 3, 2004Assignee: Institut fur Chemo- und Biosensorik Munster e.V.Inventors: Markus Meusel, Dieter Trau, Andreas Katerkamp
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Publication number: 20040014073Abstract: The present invention refers to capsules encapsulating solid particles of signal-generating organic substances and carrying on the outer surface affinity molecules for specific recognition of and binding to target molecules in a sample. The invention is directed to the use of these capsules for signal production in the optical, electrochemical or chemical detection of target molecules. To obtain a signal the signal-generating organic substances are released and dissolved. A detection method and a kit are provided.Type: ApplicationFiled: July 29, 2003Publication date: January 22, 2004Inventors: Dieter Trau, Reinhard Renneberg, Frank Caruso, Matthias Lehmann
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Publication number: 20030032199Abstract: The invention relates to devices and methods for carrying out quantitative fluorescence immunoassays using evanescent field excitation. Light from at least one light source is directed onto the boundary between two media which have differing refractive indices. The light source emits practically monochromatic light with a wavelength suitable for exciting a marking substance. The light is directed onto a boundary surface disposed between an optically transparent base plate, the refractive index of which is greater than that of the material above the boundary surface, and a receiving region for the sample. The receiving region is covered with a covering plate on the side disposed opposite the base plate, there being arranged between the base plate and covering plate at least one functional layer. A detector for detecting the fluorescent light is disposed on the same side of the base plate as the light source.Type: ApplicationFiled: August 6, 2002Publication date: February 13, 2003Applicant: Institut fur Chemo-und Biosensorik Munster e.V.Inventors: Markus Meusel, Dieter Trau, Andreas Katerkamp