Abstract: The present application relates to a chip, a preparation method therefor, and an application thereof. The chip of embodiments of the present application comprises: a substrate having a surface grafted with an amino group, the amino group being a primary amino group or a secondary amino group; and a first compound linked to the surface via the amino group, the first compound comprising the following structure, i.e., formula (1) or formula (2), wherein R1 and R2 are each independently selected from —OH or C1-C5 alkoxy, and n is 1 or 2. The chip can be used for a nucleic acid test, such as single-molecule sequencing and next-generation sequencing.

Abstract: The present invention relates to a substrate, and a preparation method therefor and the use thereof. What is referred to as the substrate has a surface, the surface comprising a polymer coating covalently attached thereto. The polymer coating comprises a polymer comprising a repeating unit A as represented by formula I and a repeating unit B as represented by formula II or formula III: wherein X is selected from —O— or —NH—, R01?, R01? and R01?? or are each independently selected from —H or a C1-C3 alkyl, R0 is selected from a C1-C10 alkyl or —(C1-C5 alkyl)-NH—NHS-PEG4, and R0 comprises at least one R02 substitution, at least one R02 substitution each independently selected from epoxy, amino, or azido, R03?, R03?, R03??, R03??, R04?, R04?, R04??, R05?, R05? and R05?? are each independently selected from —H, a C1-C3 alkyl, acylamino or ester group, L1 is selected from a C1-C3 alkylene or —C(O)—R06—C(O)—, and R06 is selected from PEG or alkyldiamine.