Abstract: A heart valve bioprosthesis (100) and a manufacturing method thereof are provided. The heart valve bioprosthesis (100) comprises: a support frame (1), valve leaflets (2) and a valve auxiliary structure. The valve leaflets (2) are connected to the valve auxiliary structure, the valve auxiliary structure is connected to the support frame (1). Both the valve leaflets (2) and the valve auxiliary structure are made of biological tissues. The support frame (1) is formed as a single component with an elastic material, and the valve auxiliary structure is preset with suturing marks. The heart valve bioprosthesis improves the biocompatibility with the human body, reduces the total height of the valve, shortens the suturing time, and decreases the possibility of thrombus or bacterial attachment thereto. In addition, the heart valve bioprosthesis is made by a simple process, exhibit excellent compliance with the cardiac tissue, and has a prolonged service life.