Abstract: P2Y1 receptor and an antagonist thereof are used in anti-depression and/or anti-anxiety disorder. It has been verified through experimental studies that the P2Y1 receptor can be used for screening for a medicine of anti-depression and/or anti-anxiety disorder, and also used for screening for depression and/or anxiety disorder, and for early warning of depression and/or anxiety disorder; and the antagonist of P2Y1 receptor has anti-depression and anti-anxiety effects which can be used to prepare a medicine of anti-depression and/or anti-anxiety disorder.

Abstract: A multi-step method for fabricating a tissue engineering bone comprises the following steps: (1) scaffold repair and pre-vascularization: fabricating a tissue engineering bone scaffold at a bone defect site, and conducting pre-vascularization; and (2) post-implantation of a seed cell for bone tissue engineering: after an inflammatory reaction period, combined with time for micro-vessel in-growth, implanting an osteogenic-induced seed cell into a tissue engineering bone scaffold at the bone defect site. In certain embodiments, the step (2) of post-implantation of a seed cell for bone tissue engineering is performed 7 to 14 days after the step (1) of scaffold repair and pre-vascularization. The method can be used to rapidly fabricate a large section of tissue engineering bone, greatly reduce usage of the seed cell for bone tissue engineering required in repair of per unit volume of bone tissue, and improve the utilization rate of the seed cell.