Abstract: A power supply mechanism for a long-stroke seat has a wire harness accommodation box having at least one accommodation space and at least one opening; a drag chain bent and accommodated in the accommodation space, where a first end of the drag chain is relatively fixed to the wire harness accommodation box, and a second end of the drag chain passes through the opening and is slidably disposed in a slideway of a lower slide rail of the seat; a wire harness threaded in the drag chain, where a first end of the wire harness passes through a first end of a wire harness channel and the opening to be connected to a power supply, and a second end of the drag chain passes through a second end of the wire harness channel to be connected to an upper slide rail of the seat.

Abstract: Compositions and methods are provided for simple, instrument-free and sensitive methods that enable rapid, point-of-care detection of nucleic acid molecules of interest. This is based on a surprising discovery that the relative efficiencies of amplification and CRISPR-based cleavage and detection can be tuned to favor amplification until sufficient amplified products are generated to enable detection. Example approaches include design of guide RNA and primers to target nonoptimal PAM sequences, or sequence-engineering Cas nucleases to reduce activities informing a ribonucleoprotein with the guide RNA or binding to or cleaving the substrate nucleic acid.