Patents by Inventor Dino De Angelis

Dino De Angelis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Hybrid molecules and their use for optically detecting changes in cellular microenvironments
    Patent number: 7094888
    Abstract: The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.
    Type: Grant
    Filed: September 30, 2003
    Date of Patent: August 22, 2006
    Assignee: Memorial Sloan-Kettering Cancer Center
    Inventors: Gero Miesenböck, Dino De Angelis, James E. Rothman
  • Hybrid molecules and their use for optically detecting changes in cellular microenvironments
    Publication number: 20040137611
    Abstract: The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.
    Type: Application
    Filed: September 30, 2003
    Publication date: July 15, 2004
    Inventors: Gero Miesenbock, Dino De Angelis, James E. Rothman
  • Hybrid molecules and their use for optically detecting changes in cellular microenvironments
    Patent number: 6670449
    Abstract: The invention relates to methods and compositions which utilize the emission of light to monitor changes in microenvironments involving cells. The invention is especially useful for monitoring exocytotic activity such as detecting quantal release of synaptic vesicles. Fusion proteins of Cypridina luciferase and synaptotagmin-I or VAMP/synaptobrevin-2 were targeted to synaptic vesicles and, upon exocytosis, formed light-emitting complexes with luciferin present in the extracellular medium. Photon emissions in the presence of a depolarizing stimulus can be observed with these systems. pH-sensitive mutants of green fluorescent protein are also provided, which are useful for visualizing exocytosis and for imaging and measuring the pH of intracellular compartments.
    Type: Grant
    Filed: February 13, 1998
    Date of Patent: December 30, 2003
    Assignee: Memorial Sloan-Kettering Cancer Center
    Inventors: Gero Miesenböck, Dino De Angelis, James E. Rothman