Abstract: The invention provides a method for homogenized coherent excitation of a sample for determining molecular structure. The method includes selecting a monochromatic coherent light, homogenizing the monochromatic coherent light; irradiating the sample with the homogenized monochromatic light; collecting the Raman scattered light to obtain a profile and analyzing the profile to obtain chemical specific signature of the sample. The invention also provides a method for obtaining two dimensional imaging of a sample using irradiation of large sample area by homogenized monochromatic light without compromise in spatial resolution. A system for homogenized coherent excitation of a sample for determining molecular structure is also provided.

Abstract: The method includes homogenizing a monochromatic coherent light source, irradiating the sample at plurality of points along all planes with the homogenized monochromatic light, collecting the molecular scattered light from all angles and planes to obtain a plurality of profile, resolving the plurality of profiles to obtain a molecular intensity maps, and reconstituting the intensity maps to obtain a three dimensional image of the sample. The system described is capable of obtaining molecular specific 3D morphology and profile of samples. The system described is capable of differentiating different chemicals or sample distribution throughout the 3D volume.