Patents by Inventor Dirk Loeffert
Dirk Loeffert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120190027Abstract: The present invention is related to normalized quantification of nucleic acids and to the normalization of quantities of nucleic acids in samples, e.g. mixtures of nucleic acids. The present invention relates to method for the normalization of the quantity of a nucleic acid to be quantified in a sample to the total quantity of nucleic acid in the sample; or to the total quantity of a specific class of nucleic acid in the sample.Type: ApplicationFiled: July 30, 2010Publication date: July 26, 2012Inventors: Dirk Loeffert, Christian Korfhage, Holger Engel
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Publication number: 20120107813Abstract: The invention relates to a method for the quantification of one or more nucleic acids in a sample. The method comprises the following steps: making a sample available which contains at least one nucleic acid to be quantified, adding an oligonucleotide probe to the sample, the oligonucleotide probe comprising a sequence which can specifically hybridize to the nucleic acid to be quantified or to a common sequence of the nucleic acids to be quantified, incubating the sample under conditions which allow the hybridization of the oligonucleotide probe to the nucleic acid(s) to be quantified, incubating the sample under conditions which allow the extension of hybridized probes, the nucleic acid(s) serving as a template in each case, removing the non-hybridized probes from the sample and quantifying the hybridized oligonucleotide probes to measure the quantity of the nucleic acid(s) to be quantified. The invention also relates to a kit for carrying out said method.Type: ApplicationFiled: March 9, 2010Publication date: May 3, 2012Inventors: Nan Fang, Andreas Missel, Dirk Löffert
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Patent number: 8129150Abstract: The invention relates to composition or a kit containing an enzyme that is reversibly inhibited by means of a chemical modification and an enzyme which is reversibly inhibited using non-covalent binding, the use of a mixture of enzymes reversibly inhibited in such a manner for processing or multiplying polynucleotides, and a method for specifically amplifying DNA by simultaneously using both types of reversibly inhibited enzymes.Type: GrantFiled: March 30, 2007Date of Patent: March 6, 2012Assignee: QIAGEN GmbHInventors: Holger Engel, Dirk Löffert, Andreas Missel, Ralf Peist
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Patent number: 8124391Abstract: The invention relates to a thermostable polymerase based on thermococcus pacificus; DNA molecules which code for one such polymerase; expression vectors; host cells; methods for producing one such polymerase and the use thereof for polymerising nucleic acid, especially in the polymerase chain reaction.Type: GrantFiled: October 5, 2001Date of Patent: February 28, 2012Assignee: Qiagen GmbHInventors: Katja Decker, Dirk Löffert, Jie Kang
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Patent number: 8076069Abstract: The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salType: GrantFiled: June 3, 2009Date of Patent: December 13, 2011Assignee: Qiagen GmbHInventors: Christian Korfhage, Friederike Wilmer, Dirk Löffert, Ralf Himmelreich, Claudia Fritz, Kathleen Rieske
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Patent number: 8067206Abstract: The present invention relates generally to the field of nucleic acid chemistry. More specifically, it relates to a method for enhancing the performance of coamplification reactions, e.g., multiplex PCR reactions.Type: GrantFiled: September 24, 2003Date of Patent: November 29, 2011Assignee: Qiagen GmbHInventors: Holger Engel, Dirk Löffert
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Publication number: 20110124050Abstract: The present invention relates to a method for synthesizing a cDNA in a sample in an enzymatic reaction, characterized in that the method comprises the steps: simultaneously providing of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide, adding of a sample comprising a ribonucleic acid and incubating the agents from the preceding steps in one or more temperature steps, which are selected so that the first enzyme and the second enzyme display activity, characterized in that additionally an amplification takes place in the same reaction mixture.Type: ApplicationFiled: February 13, 2008Publication date: May 26, 2011Inventors: Holger Engel, Subrahmanyam Yerramilli, Martin Kreutz, Dirk Löffert, Christian Korfhage
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Publication number: 20110071031Abstract: The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.Type: ApplicationFiled: August 5, 2010Publication date: March 24, 2011Applicants: QIAGEN GAITHERSBURG INC., QIAGEN GMBHInventors: Yuri KHRIPIN, Dirk Loeffert, Roland Fabis, Nadine Krueger
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Publication number: 20110053143Abstract: Methods and kits for determining load of an infectious agent in a sample are described, comprising performing at least one hybridization assay and calculating the load of the infectious agent in the sample from a detected nucleic acid. In particular, the methods and kits disclosed determine the load of human papillomavirus (HPV) in a sample.Type: ApplicationFiled: September 3, 2010Publication date: March 3, 2011Applicant: QIAGEN GAITHERSBURG INC.Inventors: Brian Lowe, Dominic O'Neil, Irina Nazarenko, Dirk Loeffert
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Publication number: 20110045542Abstract: The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.Type: ApplicationFiled: February 6, 2009Publication date: February 24, 2011Inventors: Christian Korfhage, Dirk Loeffert, Ralf Peist, Nicolas Rudinger
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Publication number: 20100323363Abstract: The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium sType: ApplicationFiled: August 24, 2010Publication date: December 23, 2010Applicant: QIAGEN GmbHInventors: Christian KORFHAGE, Friederike Wilmer, Dirk Löffert, Ralf Himmelreich, Claudia Fritz, Kathleen Rieske
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Patent number: 7838233Abstract: The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium saType: GrantFiled: April 1, 2006Date of Patent: November 23, 2010Assignee: Qiagen GmbHInventors: Christian Korfhage, Friederike Wilmer, Dirk Löffert, Ralf Himmelreich, Claudia Fritz, Kathleen Rieske
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Publication number: 20100285473Abstract: Disclosed herein are methods of amplifying a target nucleic acid in a helicase-dependent reaction. Also disclosed are methods of amplifying and detecting a target nucleic acid in a helicase-dependent reaction as well as modified detection labels to assist in the detection.Type: ApplicationFiled: January 27, 2010Publication date: November 11, 2010Inventors: John Wolff, Victoria Doseeva, Thomas Forbes, Gwynne Roth, Irina Nazarenko, Dirk Loeffert
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Publication number: 20100209912Abstract: The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salType: ApplicationFiled: June 3, 2009Publication date: August 19, 2010Applicant: QIAGEN GMBHInventors: Christian KORFHAGE, Friederike WILMER, Dirk LÖFFERT, Ralf HIMMELREICH, Claudia FRITZ, Kathleen RIESKE
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Publication number: 20100099150Abstract: The invention relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention also relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention further relates to a method for enzymatic nucleic acid synthesis comprising the steps of, (a) providing in a reaction mixture, a polymerase activity, a nucleic acid template, a zwitterionic detergent, a buffer, a salt, nucleotides and an inert protein and, (b) incubating the reaction mixture at a temperature which enables nucleic acid synthesis.Type: ApplicationFiled: March 6, 2008Publication date: April 22, 2010Inventors: Nan Fang, Dirk Löffert, Christoph Erbacher, Lars-Erik Peters
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Publication number: 20090299047Abstract: The present invention concerns a method for activating a nucleic acid for a polymerase reaction with the steps: (a) Heating a nucleic acid to a temperature of 55° C. to 80° C., (b) cooling the nucleic acid to a temperature at which a polymerase shows no substantial decrease in activity, and (c) starting the polymerase reaction by the addition of a heat-labile polymerase to the nucleic acid.Type: ApplicationFiled: September 11, 2006Publication date: December 3, 2009Applicant: QIAGEN GMBHInventors: Christian Korfhage, Dirk Löffert
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Patent number: 6300069Abstract: Novel compositions and methods useful for the generation of nucleic acids from a ribonucleic acid template and further nucleic acid replication are disclosed. It is shown that the generation and amplification of nucleic acids by methods that utilize two or more different polymerases, such as reverse transcriptase-polymerase chain reaction (RT-PCR), are dramatically more sensitive and efficient in the presence of a homopolymeric nucleic acid. Homopolymeric nucleic acids have been found to reduce or negate the inhibitory effect reverse transcriptases have on DNA polymerase activity. It is demonstrated that this inhibition-relieving effect of homopolymeric nucleic acids is general in nature; independent of the chemical species of homopolymer used, or the chemical composition of the polymerization reaction mixture.Type: GrantFiled: May 3, 1999Date of Patent: October 9, 2001Assignee: Qiagen GmbHInventors: Andreas Missel, Dirk Löffert, Jie Kang, Christian Korfhage
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Patent number: 6183998Abstract: A method for the amplification of a target nucleic acid is disclosed comprising the steps of reacting a nucleic acid with an amplification reaction mixture and a modified thermostable enzyme, wherein said modified thermostable polymerase is prepared by a reaction of a mixture of a thermostable polymerase and a chemical modifying reagent. The chemical modification reagent is an aldehyde, preferably formaldehyde. Essentially complete inactivation of the enzyme at ambient temperatures is achieved, with recovery of enzymatic activity at temperatures above 50° C.Type: GrantFiled: October 31, 1998Date of Patent: February 6, 2001Assignee: Qiagen GmbH Max-Volmer-Strasse 4Inventors: Igor Ivanov, Dirk Löffert, Jie Kang, Joachim Ribbe, Kerstin Steinert