Patents by Inventor Dmitri B. Kirpotin

Dmitri B. Kirpotin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20160138044
    Abstract: Provided are novel compositions useful for delivering nucleic acids to cells. Also provided are methods for making and using such compositions.
    Type: Application
    Filed: January 22, 2016
    Publication date: May 19, 2016
    Inventors: Mark E. Hayes, Dmitri B. Kirpotin
  • Publication number: 20160122411
    Abstract: Provided herein are fusion proteins comprising a first domain that specifically binds to the extracellular domain of a growth factor receptor, and a second domain that specifically binds to a cartilage matrix component, and pharmaceutical composition comprising these fusion proteins. Methods of treating musculoskeletal diseases using the fusion proteins and pharmaceutical composition disclosed herein are also provided.
    Type: Application
    Filed: March 28, 2014
    Publication date: May 5, 2016
    Applicant: Merrimack Pharmaceuticals, Inc.
    Inventors: Emily Florine, Dmitri B. Kirpotin, Paul Kopesky, Alexey Lugovskoy, Rachel Rennard, Birait Schoeberl
  • Publication number: 20150140069
    Abstract: Provided herein is a process for preparing a microparticulate complex of a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible organic solvent. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component, and a composition comprising water and the microparticulate complex. Further provided is a method of delivery of a nucleicacid-like component to a cell or to a patient by contacting the cell with or administering to the patient a composition comprising water and the microparticulate complex described herein. Still further, a charge-changing composition represented by the formula A-X—B is provided.
    Type: Application
    Filed: September 30, 2014
    Publication date: May 21, 2015
    Inventors: Keelung Hong, Wei Wen Zheng, Daryl C. Drummond, Dmitri B. Kirpotin, Mark Eamon Hayes
  • Publication number: 20150093328
    Abstract: The present invention relates to a novel composition useful in targeted diagnostic and/or therapy of a target site, such as cancerous tissue. The composition and methods disclosed herein find particular use in diagnosing and imaging cancerous tissue. The present invention provides a new diagnostic tool for the utilization of positron emission tomography (PET) imaging technique.
    Type: Application
    Filed: April 17, 2013
    Publication date: April 2, 2015
    Applicant: MERRIMACK PHARMACEUTICALS, INC.
    Inventors: Daryl C. Drummond, Dmitri B. Kirpotin, Thomas Wickham, Bart S. Hendricks, Samuel Agresta, Helen Lee
  • Patent number: 8883200
    Abstract: Provided herein is a process for preparing a microparticulate complex of a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible organic solvent. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component, and a composition comprising water and the microparticulate complex. Further provided is a method of delivery of a nucleic acid-like component to a cell or to a patient by contacting the cell with or administering to the patient a composition comprising water and the microparticulate complex described herein. Still further, a charge-changing composition represented by the formula A-X—B is provided.
    Type: Grant
    Filed: July 11, 2013
    Date of Patent: November 11, 2014
    Assignee: Sutter West Bay Hospitals
    Inventors: Keelung Hong, Wei Wen Zheng, Daryl C. Drummond, Dmitri B. Kirpotin, Mark Eamon Hayes
  • Publication number: 20140017300
    Abstract: Provided herein is a process for preparing a microparticulate complex of a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible organic solvent. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component, and a composition comprising water and the microparticulate complex. Further provided is a method of delivery of a nucleic acid-like component to a cell or to a patient by contacting the cell with or administering to the patient a composition comprising water and the microparticulate complex described herein. Still further, a charge-changing composition represented by the formula A-X—B is provided.
    Type: Application
    Filed: July 11, 2013
    Publication date: January 16, 2014
    Applicant: Sutter West bay Hospital, DBA California Pacific Medical Center
    Inventors: Keelung Hong, Wei-Wen Zheng, Daryl C. Drummond, Dmitri B. Kirpotin, Mark Eamon Hayes
  • Patent number: 8496961
    Abstract: A process for preparing a microparticulate complex is provided. The process comprises (a) combining a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible, organic solvent to form a mixture wherein the PFC and the NAC are independently molecularly or micellarly soluble in the aqueous/organic solvent system, and (b) reducing the amount of the organic solvent in the mixture. This effects formation of the microparticulate complex of the NAC and the PFC. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component forming an approximately spherical particle, wherein the particle-forming component encloses an interior of the particle containing the nucleic acid-like component and the so-enclosed interior volume has less than about 50% (preferably less than 20%) of the volume containing free water.
    Type: Grant
    Filed: May 15, 2003
    Date of Patent: July 30, 2013
    Assignee: Sutter West Bay Hospital
    Inventors: Keelung Hong, Wei-Wen Zheng, Daryl C. Drummond, Dmitri B. Kirpotin, Mark Eamon Hayes
  • Publication number: 20110104261
    Abstract: The present invention provides for the development of endocytosis-sensitive probes, and a remote method for measuring cellular endocytosis. These probes are based on the reduced water permeability of a nanoparticle or liposomal delivery system, and inherent degradability or disruption of barrier integrity upon endocytosis. The invention also provides for liposomes having combined therapeutic and diagnostic utilities by co-encapsulating ionically coupled diagnostic and therapeutic agents, in one embodiment, by a method using anionic chelators to prepare electrochemical gradients for loading of amphipathic therapeutic bases into liposomes already encapsulating an imaging agent. The invention provides for imaging of therapeutic liposomes by inserting a lipopolymer anchored, remotely sensing reporter molecules into liposomal lipid layer. The invention allows for an integrated delivery system capable of imaging molecular fingerprints in diseased tissues, treatment, and treatment monitoring.
    Type: Application
    Filed: January 7, 2011
    Publication date: May 5, 2011
    Inventors: Daryl C. Drummond, Keelung Hong, Dmitri B. Kirpotin
  • Publication number: 20100008978
    Abstract: This invention provides antibodies that have improved affinity for the epidermal growth factor receptor (EGFR). In addition, this invention provides microparticles and nanoparticles comprising a plurality of EGFR affinity moieties that are effectively internalized by cells expressing an EGFR.
    Type: Application
    Filed: May 8, 2009
    Publication date: January 14, 2010
    Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, HERMES BIOSCIENCES, INC.
    Inventors: DARYL C. DRUMMOND, DMITRI B. KIRPOTIN, JAMES D. MARKS, YU ZHOU
  • Publication number: 20090171077
    Abstract: A process for preparing a microparticulate complex is provided. The process comprises (a) combining a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible, organic solvent to form a mixture wherein the PFC and the NAC are independently molecularly or micellarly soluble in the aqueous/organic solvent system, and (b) reducing the amount of the organic solvent in the mixture. This effects formation of the microparticulate complex of the NAC and the PFC. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component forming an approximately spherical particle, wherein the particle-forming component encloses an interior of the particle containing the nucleic acid-like component and the so-enclosed interior volume has less than about 50% (preferably less than 20%) of the volume containing free water.
    Type: Application
    Filed: March 10, 2009
    Publication date: July 2, 2009
    Inventors: Keelung Hong, Wei-Wen Zheng, Daryl C. Drummond, Dmitri B. Kirpotin, Mark Eamon Hayes
  • Publication number: 20090155272
    Abstract: The disclosure pertains to pharmaceuticals, more specifically, to targeted diagnostic and therapeutic formulations and ligands thereof. Such methods and composition comprise antigens that are post-translationally modified compared to antigens found on a normal cell phenotype. Also provided are ligands that bind to such post-translationally modified antigens.
    Type: Application
    Filed: September 29, 2006
    Publication date: June 18, 2009
    Inventors: John L. Muschler, Dmitri B. Kirpotin, Daryl C. Drummond
  • Patent number: 7022336
    Abstract: The invention provides methods of attaching proteins to lipidic microparticles with efficiencies of at least 77%. The proteins are attached to linker molecules comprising a hydrophilic domain and a hydrophobic domain. The proteins can be antibodies, antibody fragments, hormones, growth factors, enzymes, or nucleic acid binding proteins, or other proteins. The proteins can be chemically conjugated to the linker molecules, or they can be fused to the linker molecules by recombinant techniques.
    Type: Grant
    Filed: May 17, 2004
    Date of Patent: April 4, 2006
    Assignee: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
  • Publication number: 20040209366
    Abstract: The invention provides methods of attaching proteins to lipidic microparticles with efficiencies of at least 77%. The proteins are attached to linker molecules comprising a hydrophilic domain and a hydrophobic domain. The proteins can be antibodies, antibody fragments, hormones, growth factors, enzymes, or nucleic acid binding proteins, or other proteins. The proteins can be chemically conjugated to the linker molecules, or they can be fused to the linker molecules by recombinant techniques.
    Type: Application
    Filed: May 17, 2004
    Publication date: October 21, 2004
    Applicant: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
  • Patent number: 6803053
    Abstract: The invention provides lipidic microparticles stably associated with at least two different targeting moieties, which targeting moieties are attached to linker molecules comprising a hydrophilic domain and a hydrophobic domain. The targeting moieties can be antibodies, antibody fragments, hormones, growth factors, enzymes, or nucleic acid binding proteins, or other proteins. The targeting moieties can be chemically conjugated to the linker molecules, or they can be fused by recombinant techniques.
    Type: Grant
    Filed: June 21, 2002
    Date of Patent: October 12, 2004
    Assignee: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
  • Publication number: 20040037874
    Abstract: A process for preparing a microparticulate complex is provided. The process comprises (a) combining a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible, organic solvent to form a mixture wherein the PFC and the NAC are independently molecularly or micellarly soluble in the aqueous/organic solvent system, and (b) reducing the amount of the organic solvent in the mixture. This effects formation of the microparticulate complex of the NAC and the PFC. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component forming an approximately spherical particle, wherein the particle-forming component encloses an interior of the particle containing the nucleic acid-like component and the so-enclosed interior volume has less than about 50% (preferably less than 20%) of the volume containing free water.
    Type: Application
    Filed: May 15, 2003
    Publication date: February 26, 2004
    Inventors: Keelung Hong, Wei-Wen Zheng, Daryl C. Drummond, Dmitri B. Kirpotin, Mark Eamon Hayes
  • Patent number: 6528087
    Abstract: The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab′ fragment attached to a hydrophilic polymer.
    Type: Grant
    Filed: January 16, 2001
    Date of Patent: March 4, 2003
    Assignee: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
  • Publication number: 20030003143
    Abstract: The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab′ fragment attached to a hydrophilic polymer.
    Type: Application
    Filed: June 21, 2002
    Publication date: January 2, 2003
    Applicant: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
  • Publication number: 20020001612
    Abstract: The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab′ fragment attached to a hydrophilic polymer.
    Type: Application
    Filed: January 16, 2001
    Publication date: January 3, 2002
    Applicant: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin
  • Patent number: 6210707
    Abstract: The present invention provides for lipid:nucleic acid complexes that have increased shelf life and high transfection activity in vivo following intravenous injection, and methods of preparing such complexes. The methods generally involve contacting a nucleic acid with an organic polycation to produce a condensed nucleic acid, and then combining the condensed nucleic acid with a lipid comprising an amphiphilic cationic lipid to produce the lipid:nucleic acid complex. This complex can be further stabilized by the addition of a hydrophilic polymer attached to hydrophobic side chains. The complex can also be made specific for specific cells, by incorporating a targeting moiety such as an Fab′ fragment attached to a hydrophilic polymer.
    Type: Grant
    Filed: May 12, 1998
    Date of Patent: April 3, 2001
    Assignee: The Regents of the University of California
    Inventors: Demetrios Papahadjopoulos, Keelung Hong, Weiwen Zheng, Dmitri B. Kirpotin