Patents by Inventor Dmitriy Gremyachinskiy

Dmitriy Gremyachinskiy has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240209432
    Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.
    Type: Application
    Filed: June 28, 2023
    Publication date: June 27, 2024
    Applicant: Roche Sequencing Solutions, Inc.
    Inventors: Frank BERGMANN, Christoph SEIDEL, Andrew TRANS, Dmitriy GREMYACHINSKIY, Hannes KUCHELMEISTER, Lars HILLRINGHAUS
  • Patent number: 11993807
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Grant
    Filed: March 7, 2023
    Date of Patent: May 28, 2024
    Assignee: Nautilus Subsidiary, Inc.
    Inventors: Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
  • Publication number: 20240166698
    Abstract: The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein ?-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.
    Type: Application
    Filed: November 16, 2023
    Publication date: May 23, 2024
    Applicant: Roche Sequencing Solutions, Inc.
    Inventors: Dmitriy GREMYACHINSKIY, Christos TZITZILONIS
  • Publication number: 20240018574
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Application
    Filed: June 2, 2023
    Publication date: January 18, 2024
    Inventors: Tural AKSEL, Torri RINKER, Markus BURNS, Michael DORWART, Rachel GALIMIDI, Dmitriy GREMYACHINSKIY, Stephen HENDRICKS, Elvis IKWA, Gregory KAPP, Joshua Simon KLEIN, Julia ROBINSON, Cassandra STAWICKI, Sonal TONAPI, Parag MALLICK
  • Patent number: 11866464
    Abstract: The present disclosure relates to relates methods and associated compositions that provide fast, efficient site-selective conjugation of a protein, such as the pore-forming protein ?-hemolysin, to a biomolecule, such as a DNA polymerase, and the use of such site-selective protein-biomolecule conjugates in nanopore devices and methods.
    Type: Grant
    Filed: April 9, 2021
    Date of Patent: January 9, 2024
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Dmitriy Gremyachinskiy, Christos Tzitzilonis
  • Publication number: 20230407391
    Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
    Type: Application
    Filed: June 14, 2023
    Publication date: December 21, 2023
    Inventors: Steven M. MENCHEN, Alan BLANCHARD, Luisa ANDRUZZI, Shaheer KHAN, Dmitriy GREMYACHINSKIY, Alfred LUI, Craig STOLARCZYK, Tanya SOKOLSKY, Prasanna Krishnan THWAR
  • Publication number: 20230340014
    Abstract: The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.
    Type: Application
    Filed: May 16, 2023
    Publication date: October 26, 2023
    Inventors: Dmitriy GREMYACHINSKIY, Rachel GALIMIDI, Parag MALLICK, Sujal M. PATEL
  • Publication number: 20230272459
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Application
    Filed: March 7, 2023
    Publication date: August 31, 2023
    Inventors: Tural AKSEL, Torri RINKER, Markus BURNS, Michael DORWART, Rachel GALIMIDI, Dmitriy GREMYACHINSKIY, Stephen HENDRICKS, Elvis IKWA, Gregory KAPP, Joshua Simon KLEIN, Julia ROBINSON, Cassandra STAWICKI, Sonal TONAPI, Parag MALLICK
  • Patent number: 11739378
    Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.
    Type: Grant
    Filed: March 15, 2021
    Date of Patent: August 29, 2023
    Assignees: Roche Sequencing Solutions, Inc., Roche Molecular Systems, Inc.
    Inventors: Frank Bergmann, Christoph Seidel, Andrew Trans, Dmitriy Gremyachinskiy, Hannes Kuchelmeister, Lars Hillringhaus
  • Publication number: 20230257416
    Abstract: The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.
    Type: Application
    Filed: March 9, 2023
    Publication date: August 17, 2023
    Inventors: Dmitriy GREMYACHINSKIY, Rachel GALIMIDI, Parag MALLICK, Sujal M. PATEL
  • Patent number: 11702696
    Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.
    Type: Grant
    Filed: July 24, 2020
    Date of Patent: July 18, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Steven M. Menchen, Alan Blanchard, Luisa Andruzzi, Shaheer Khan, Dmitriy Gremyachinskiy, Alfred Lui, Craig Stolarczyk, Tanya Sokolsky, Prasanna Krishnan Thwar
  • Patent number: 11692217
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Grant
    Filed: November 10, 2021
    Date of Patent: July 4, 2023
    Assignee: Nautilus Subsidiary, Inc.
    Inventors: Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
  • Publication number: 20230175046
    Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.
    Type: Application
    Filed: February 1, 2023
    Publication date: June 8, 2023
    Inventors: David LIGHT, Wolfgang HINZ, Ronald CICERO, Christina INMAN, Paul KENNEY, Alexander MASTROIANNI, Roman ROZHKOV, Yufang WANG, Jeremy GRAY, Marc GLAZER, Dmitriy GREMYACHINSKIY
  • Patent number: 11603383
    Abstract: The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.
    Type: Grant
    Filed: March 9, 2022
    Date of Patent: March 14, 2023
    Assignee: Nautilus Biotechnology, Inc.
    Inventors: Dmitriy Gremyachinskiy, Rachel Galimidi, Parag Mallick, Sujal M. Patel
  • Patent number: 11578356
    Abstract: A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.
    Type: Grant
    Filed: July 24, 2020
    Date of Patent: February 14, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: David Light, Wolfgang Hinz, Ronald Cicero, Christina Inman, Paul Kenney, Alexander Mastroianni, Roman Rozhkov, Yufang Wang, Jeremy Gray, Marc Glazer, Dmitriy Gremyachinskiy
  • Publication number: 20220204549
    Abstract: The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.
    Type: Application
    Filed: March 9, 2022
    Publication date: June 30, 2022
    Inventors: Dmitriy GREMYACHINSKIY, Rachel GALIMIDI, Parag MALLICK, Sujal M. PATEL
  • Publication number: 20220162684
    Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.
    Type: Application
    Filed: November 10, 2021
    Publication date: May 26, 2022
    Inventors: Tural AKSEL, Torri RINKER, Markus BURNS, Michael DORWART, Rachel GALIMIDI, Dmitriy GREMYACHINSKIY, Stephen HENDRICKS, Elvis IKWA, Gregory KAPP, Joshua KLEIN, Julia ROBINSON, Cassandra STAWICKI, Sonal TONAPI, Parag MALLICK
  • Publication number: 20220090189
    Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.
    Type: Application
    Filed: March 15, 2021
    Publication date: March 24, 2022
    Applicant: Roche Sequencing Solutions, Inc.
    Inventors: Frank BERGMANN, Christoph SEIDEL, Andrew TRANS, Dmitriy GREMYACHINSKIY, Hannes KUCHELMEISTER, Lars HILLRINGHAUS
  • Publication number: 20220017567
    Abstract: The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.
    Type: Application
    Filed: October 7, 2021
    Publication date: January 20, 2022
    Inventors: Dmitriy GREMYACHINSKIY, Rachel GALIMIDI, Parag MALLICK, Sujal M. PATEL
  • Patent number: 11203612
    Abstract: The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.
    Type: Grant
    Filed: October 2, 2020
    Date of Patent: December 21, 2021
    Assignee: Nautilus Biotechnology, Inc.
    Inventors: Dmitriy Gremyachinskiy, Rachel Galimidi, Parag Mallick, Sujal M. Patel