Abstract: Methods of amplifying specific fragments of DNA or RNA with the aid of a polymerase chain reaction in real time are disclosed. The methods include providing a first oligonucleotide primer that comprises a donor fluorescent dye and a second oligonucleotide primer that comprises an acceptor fluorescent dye; allowing the primers to anneal to a target nucleic acid at positions abutting to each other; carrying out a polymerase chain reaction which allows for a fluorescent resonant transfer of energy between the donor dye and the acceptor dye; detecting an increase of fluorescent emission of the acceptor dye; and correlating the increase in the emission of the acceptor dye with the accumulation of the specific fragment of DNA or RNA. In one embodiment, the primers comprise a fluorescent dye and a universal quencher.

Abstract: The present invention relates to methods using HIPK1 sequences for use in diagnosis and treatment of lymphoma and leukemia. In, addition, the present invention describes the use of these compositions for use in screening methods.