Abstract: The invention is directed to a method for determining the sequence of immune receptors expressed in target cells provided from a sample comprising target and non-target cells suspended in a fluid, especially TCR and BCR receptors. The assay has advantages over conventional RNA isolation-based approach. Cells are magnetically enriched on the column, and then RT reaction is carried out without eluting the cells, using the column as a reaction tube. Quantification of TCR libraries is produced with the standard workflow (with RNA isolation), and with on-column cDNA synthesis approach.

Abstract: Nucleic acid compositions encoding mutants of wild-type chromo/fluoroproteins whose chromo/fluorescent properties have been interconverted, as well as the proteins encoded the same, are provided. Also provided are methods for interconverting chromoproteins to fluorescent proteins, and vice versa. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Abstract: Kindling fluorescent protein compositions and nucleic acids encoding the same, as well as methods for using the same, are provided. The kindling fluorescent proteins are characterized in that they become brightly fluorescent proteins, from an initial non-fluorescent or low fluorescent state, upon exposure to a kindling stimulus, which fluorescent state may be reversible or irreversible. The subject protein/nucleic acid compositions find use in labeling protocols, e.g., in labeling proteins, organelles, cells and organisms, etc., in a variety of different types of applications. Also provided are systems and kits for use in practicing such applications.