Abstract: A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers.

Abstract: Provided herein are methods of processing in-situ (spatial) molecular data from comics measurements of solid tissues to identify complex, network-level biomarkers using deep learning based on location, molecular analyte, biological interactions, and patient metadata to classify disease states, identify drug targets, and predict therapeutic response and outcomes.

Abstract: A method for dissecting and collecting one or more cells from a tissue sample fixed to an inner surface of a microfluidic device is described. The tissue sample is in fluid communication with a channel having an inlet end and an outlet end defined by the microfluidic device. The method comprises flowing a first fluid through the channel with a fluid flow from the inlet end to the outlet end; powering a laser to direct laser energy into the channel to impinge upon the first fluid proximate a first region of the tissue sample and cause fluid cavitation to thereby ablate a first set of one or more cells from the tissue sample; and collecting the first set of one or more cells within a first sample container coupled to the outlet end.

Abstract: A method for analyzing cells through measurement of live-cell parameters followed by measurement of gene and protein expression is disclosed herein. The method comprises measuring one or more live-cell parameters for a plurality of cells contained in at least one liquid in a plurality of isolated microchambers of a microarray device. The method further comprises removing a lid bounding the plurality of isolated microchambers. The method further comprises microdispensing a quantity of lysate into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of reverse transcription polymerase chain reaction mix into each microchamber of the plurality of isolated microchambers. The method further comprises microdispensing a quantity of oil into each microchamber of the plurality of isolated microchambers.

Abstract: A method for dissecting and collecting one or more cells from a tissue sample fixed to an inner surface of a microfluidic device is described. The tissue sample is in fluid communication with a channel having an inlet end and an outlet end defined by the microfluidic device. The method comprises flowing a first fluid through the channel with a fluid flow from the inlet end to the outlet end; powering a laser to direct laser energy into the channel to impinge upon the first fluid proximate a first region of the tissue sample and cause fluid cavitation to thereby ablate a first set of one or more cells from the tissue sample; and collecting the first set of one or more cells within a first sample container coupled to the outlet end.

Abstract: Methods and apparatuses are provided for extraction and collection of sets of one or more cells from a formalin-fixed paraffin embedded tissue sample. A laser is used to remove sets of one or more cells from an immobilized sample within a microfluidic device, and a fluid flow element washes the removed set of one or more cells away from the immobilized sample, preferably to be collected in a downstream sample collection device. Morphological and/or positional information may be preserved to permit at least one technique (e.g., amplification, sequencing, and/or analytical, optionally performed after cells are lysed downstream of the microfluidic device) to be related to the original spatial location of the set of one or more cells in the immobilized sample.

Abstract: Methods and apparatuses are provided for extraction and collection of sets of one or more cells from a formalin-fixed paraffin embedded tissue sample. A laser is used to remove sets of one or more cells from an immobilized sample within a microfluidic device, and a fluid flow element washes the removed set of one or more cells away from the immobilized sample, preferably to be collected in a downstream sample collection device. Morphological and/or positional information may be preserved to permit at least one technique (e.g., amplification, sequencing, and/or analytical, optionally performed after cells are lysed downstream of the microfluidic device) to be related to the original spatial location of the set of one or more cells in the immobilized sample.