Abstract: The present invention relates to a method of culturing a strain producing botulinum toxin for increasing botulinum toxin productivity, and a method of effectively producing botulinum toxin by culturing the strain by the method. The culture method of the present invention has the advantage of enabling commercial productivity by increasing botulinum toxin productivity 10-fold or more.

Abstract: The present invention relates to a method for manufacturing the promotional tab end of a can so as to be able to use the end thereof for promotional use, thereby increasing the value of a product by printing various designs and characters on the top surface of the tab. Said tab is coupled to the top of the end of the lid of a can, thereby functioning as the opening portion. Said method can subsequently enable the bottom surface of the tab to be marked for gift reward events to expedite sales by printing various designs and characters and applying serial codes or random codes thereon. More particularly, when a pitch roll is operated for tab coil printing, a number of pitch holes transferred to a pitch hole sensor are transferred via a pitch roll in a number of pitch holes equal to that to be counted so as to maintain an accurate tab coil transfer distance.

Abstract: The present invention relates to a method for manufacturing a design tab end using a pilot hole of a tab coil. The method includes punching a pilot hole on a tab coil in the same pitch as that of tab forming pitch of a conversion press prior to design printing, washing and drying the tab coil, inputting the tab coil into a printer according to the pitch of the pilot hole, detecting the pilot hole of the tab coil inputted into the printer, printing the design on the tab coil using a print roll which is controlled by the color controller, pulling and winding the design-printed tab coil according to the pitch of the pilot hole, loading the design tab coil on an uncoiler of a conversion press, tab forming the design tab and attaching the design tab onto a shell supplied into the conversion press.

Abstract: The present invention relates to a method for manufacturing a design tab end of a beverage can, in which a base color is painted on an aluminum tab coil before forming a tab, and a design is printed on the surface of the tab coil on which the base color has been painted, thereby making the color of the tab gorgeous and raising the value of the product.

Abstract: The present invention relates to a method for manufacturing a designed tab end using a pilot hole of a tab coil.

Abstract: The present invention relates to a method for manufacturing a design tab end of a beverage can, in which a base color is painted on an aluminum tab coil before forming a tab, and a design is printed on the surface of the tab coil on which the base color has been painted, thereby making the color of the tab gorgeous and raising the value of the product.