Patents by Inventor Domenico Valerio
Domenico Valerio has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12168938Abstract: A turbine vane assembly for a gas turbine engine is disclosed herein. The turbine vane assembly includes a turbine vane including a leading edge, a pressure edge, a suction edge, and a trailing edge, a core defined by the turbine vane, an outer platform end wall connected to the turbine vane, the outer platform end wall defining an interior space, the interior space being in fluid communication with the core, and a plurality of cooling holes formed in the turbine vane, the plurality of cooling holes being in fluid communication with the core.Type: GrantFiled: August 30, 2023Date of Patent: December 17, 2024Assignee: RTX CORPORATIONInventors: Jeremy B. Fredette, Robin Michael Patrick Prenter, Dominic J. Mongillo, Jr., Christopher Parent, Vladimir Skidelsky, Domenico Valerio
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Publication number: 20240218796Abstract: A turbine vane assembly for a gas turbine engine is disclosed herein. The turbine vane assembly includes a turbine vane including a leading edge, a pressure edge, a suction edge, and a trailing edge, a core defined by the turbine vane, an outer platform end wall connected to the turbine vane, the outer platform end wall defining an interior space, the interior space being in fluid communication with the core, and a plurality of cooling holes formed in the turbine vane, the plurality of cooling holes being in fluid communication with the core.Type: ApplicationFiled: August 30, 2023Publication date: July 4, 2024Applicant: RTX CorporationInventors: Jeremy B. FREDETTE, Robin Michael Patrick PRENTER, Dominic J. MONGILLO, JR., Christopher PARENT, Vladimir SKIDELSKY, Domenico VALERIO
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Publication number: 20230285541Abstract: The disclosure provides methods and compositions for increasing immunity against coronaviruses, in particular highly pathogenic coronaviruses. Compositions are provided comprising peptides comprising at least a part of the S2 ectodomain of the S (spike) protein from at least one human coronaviruses (HCoV) selected from HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1, as well as compositions comprising nucleic acid molecules encoding at least a part of the S2 ectodomain of the S (spike) protein from at least one human coronaviruses (HCoV) selected from HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1. Compositions described herein are particularly useful as vaccines, in particular against highly pathogenic coronaviruses such as SARS-CoV-1, MERS-CoV and/or SARS-CoV-2 as well as cross-species transmission of typically non-human coronaviruses.Type: ApplicationFiled: July 20, 2021Publication date: September 14, 2023Inventors: Domenico VALERIO, Jaap GOUDSMIT, Stefan Frederik Franciscus VERLINDEN
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Patent number: 8236293Abstract: Cells capable of at least, in part, complementing adenovirus an adenovirus defective in E2A function. Such cells include a nucleic acid-encoding adenovirus E2A or a functional part, derivative, temperature-sensitive mutation and/or analogue thereof, integrated into the cell's genome. Methods for producing an adenovirus particle/vector with a functional deletion of E2A are also disclosed. Such methods involve providing a cell with the functionally deleted adenovirus vector, culturing the cell, and harvesting viral particles. The functional deletion may comprise a deletion in E2A. The nucleic acid-encoding E2A in the cell's genome may lack sequence overlap with the vector, preventing formation of a replication-competent adenovirus or restoration of E2A function. The adenovirus vector may further include a functional deletion in the E1-region.Type: GrantFiled: May 19, 2005Date of Patent: August 7, 2012Assignee: Crucell Holland B.V.Inventors: Frits J. Fallaux, Robert C. Hoeben, Abraham Bout, Domenico Valerio, Alex J. van der Eb, Govert Schoutten
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Patent number: 8172533Abstract: A cooled airfoil includes a concave pressure wall extending radially from a base to a tip of the airfoil, a convex suction wall connected to the concave pressure wall at a leading edge and a trailing edge spaced axially from the leading edge, and a plurality of cooling channels formed between the concave pressure wall and the convex suction wall and configured to receive a cooling fluid supply from the base of the airfoil. The cooling channels include a leading edge channel extending radially from the base toward the tip, a trailing edge channel extending radially from the base toward the tip and in flow communication with a plurality of trailing edge apertures adapted to exhaust cooling fluid to the exterior of the airfoil, a serpentine cooling circuit including a plurality of channels, and a dedicated up-pass channel extending radially from the base toward the tip between the leading edge channel and the forward most channel of the plurality of channels in the serpentine cooling circuit.Type: GrantFiled: May 14, 2008Date of Patent: May 8, 2012Assignee: United Technologies CorporationInventors: Sandra S. Pinero, Edward F. Pietraszkiewicz, Bryan P. Dube, Stephen J. Yee, Ryan Shepard Levy, Mohamed Hassan, Domenico Valerio, Scott D. Hartmann
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Patent number: 7781208Abstract: A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell.Type: GrantFiled: February 7, 2006Date of Patent: August 24, 2010Assignee: Crucell Holland B.V.Inventors: Domenico Valerio, Victor W. Van Beusechem
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Publication number: 20090285684Abstract: A cooled airfoil includes a concave pressure wall extending radially from a base to a tip of the airfoil, a convex suction wall connected to the concave pressure wall at a leading edge and a trailing edge spaced axially from the leading edge, and a plurality of cooling channels formed between the concave pressure wall and the convex suction wall and configured to receive a cooling fluid supply from the base of the airfoil. The cooling channels include a leading edge channel extending radially from the base toward the tip, a trailing edge channel extending radially from the base toward the tip and in flow communication with a plurality of trailing edge apertures adapted to exhaust cooling fluid to the exterior of the airfoil, a serpentine cooling circuit including a plurality of channels, and a dedicated up-pass channel extending radially from the base toward the tip between the leading edge channel and the forward most channel of the plurality of channels in the serpentine cooling circuit.Type: ApplicationFiled: May 14, 2008Publication date: November 19, 2009Applicant: United Technologies CorporationInventors: Sandra S. Pinero, Edward F. Pietraszkiewicz, Bryan P. Dube, Stephen J. Yee, Ryan Shepard Levy, Mohamed Hassan, Domenico Valerio, Scott D. Hartmann
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Publication number: 20090023196Abstract: Presented are ways to address the problem of replication-competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large scale production of recombinant adenoviruses. A system for use with the invention produces replication-defective adenovirus. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication-competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred.Type: ApplicationFiled: July 16, 2007Publication date: January 22, 2009Inventors: Frits J. Fallaux, Robert C. Hoeben, Alex Jan van der Eb, Abraham Bout, Domenico Valerio
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Publication number: 20080138901Abstract: Presented are ways to address the problem of replication-competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large scale production of recombinant adenoviruses. A described system produces replication-defective adenovirus. The system includes a primary cell containing a nucleotide derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleotide is derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication-competent adenovirus in the primary cell into which the isolated recombinant nucleotide is to be transferred.Type: ApplicationFiled: September 11, 2007Publication date: June 12, 2008Applicant: Crucell Holland B.V.Inventors: Frits J. Fallaux, Robert C. Hoeben, Alex J. van der Eb, Abraham Bout, Domenico Valerio
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Publication number: 20060246569Abstract: Presented are ways to address the problem of replication-competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large scale production of recombinant adenoviruses. A described system produces replication-defective adenovirus. The system includes a primary cell containing a nucleotide derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleotide is derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication-competent adenovirus in the primary cell into which the isolated recombinant nucleotide is to be transferred.Type: ApplicationFiled: July 12, 2006Publication date: November 2, 2006Applicant: Crucell Holland B.V.Inventors: Frits Fallaux, Robert Hoeben, Alex van der Eb, Abraham Bout, Domenico Valerio
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Patent number: 7105346Abstract: Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large scale production of recombinant adenoviruses. A system for use with the invention produces replication defective adenovirus. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred.Type: GrantFiled: April 18, 2002Date of Patent: September 12, 2006Assignee: Crucell Holland B.V.Inventors: Frits J. Fallaux, Robert C. Hoeben, Alex Jan van der Eb, Abraham Bout, Domenico Valerio
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Publication number: 20060134123Abstract: A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell.Type: ApplicationFiled: February 7, 2006Publication date: June 22, 2006Inventors: Domenico Valerio, Victor Van Beusechem
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Patent number: 7052881Abstract: Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large-scale production of recombinant adenoviruses. A system for use with the invention produces replication-defective adenovirus. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred.Type: GrantFiled: May 20, 2004Date of Patent: May 30, 2006Assignee: Crucell Holland B.V.Inventors: Frits J. Fallaux, Robert C. Hoeben, Alex Jan van der Eb, Abraham Bout, Domenico Valerio
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Patent number: 7033834Abstract: A method for producing viral gene delivery vehicles which can be transferred to pre-selected cell types by using targeting conjugates. The gene delivery vehicles comprise: 1) the gene of interest; and 2) a viral capsid or envelope carrying a member of a specific binding pair, the counterpart of which is not directly associated with the surface of the target cell. These vehicles can be rendered unable to bind to their natural cell receptor. The targeting conjugates include the counterpart member of the specific binding pair, linked to a targeting moiety which is a cell-type specific ligand (or fragments thereof). The number of the specific binding pair present on the viral vehicles can be, for example, an immunoglobulin binding moiety (e.g., capable of binding to a Fc fragment, protein A, protein G, FcR or an anti-Ig antibody), or biotin, avidin or streptavidin. The virus' outer membrane or capsid may contain a substance which mediates entrance of the gene delivery vehicle into the target cell.Type: GrantFiled: July 10, 2001Date of Patent: April 25, 2006Assignee: Crucell Holland B.V.Inventors: Domenico Valerio, Victor Willem Van Beusechem
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Publication number: 20050260596Abstract: Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large scale production of recombinant adenoviruses. A system for use with the invention produces replication-defective adenovirus. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred.Type: ApplicationFiled: July 11, 2003Publication date: November 24, 2005Inventors: Frits Fallaux, Robert Hoeben, Alex van der Eb, Abraham Bout, Domenico Valerio
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Publication number: 20050221492Abstract: Cells capable of at least, in part, complementing adenovirus an adenovirus defective in E2A function. Such cells include a nucleic acid-encoding adenovirus E2A or a functional part, derivative, temperature-sensitive mutation and/or analogue thereof, integrated into the cell's genome. Methods for producing an adenovirus particle/vector with a functional deletion of E2A are also disclosed. Such methods involve providing a cell with the functionally deleted adenovirus vector, culturing the cell, and harvesting viral particles. The functional deletion may comprise a deletion in E2A. The nucleic acid-encoding E2A in the cell's genome may lack sequence overlap with the vector, preventing formation of a replication-competent adenovirus or restoration of E2A function. The adenovirus vector may further include a functional deletion in the E1-region.Type: ApplicationFiled: May 19, 2005Publication date: October 6, 2005Applicant: Crucell Holland B.V.Inventors: Frits Fallaux, Robert Hoeben, Abraham Bout, Domenico Valerio, Alex Van Der Eb
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Publication number: 20040228843Abstract: Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector are suited for large-scale production of recombinant adenoviruses. A system for use with the invention produces replication-defective adenovirus. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, has at least one functional encapsidation signal and at least one functional Inverted Terminal Repeat, and lacks overlapping sequences with the nucleic acid of the cell. Otherwise, the overlapping sequences would enable homologous recombination leading to replication competent adenovirus in the primary cell into which the isolated recombinant nucleic acid molecule is to be transferred.Type: ApplicationFiled: May 20, 2004Publication date: November 18, 2004Inventors: Frits Jacobus Fallaux, Robert Cornelis Hoeben, Alex Jan van der Eb, Abraham Bout, Domenico Valerio
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Patent number: 6783980Abstract: Presented are ways to address the problem of replication competent adenovirus in adenoviral production for use with, for example, gene therapy. Packaging cells having no overlapping sequences with a selected vector and are suited for large scale production of recombinant adenoviruses. A system for use with the invention produces adenovirus incapable of replicating. The system includes a primary cell containing a nucleic acid based on or derived from adenovirus and an isolated recombinant nucleic acid molecule for transfer into the primary cell. The isolated recombinant nucleic acid molecule is based on or derived from an adenovirus, and further has at least one functional encapsidating signal, and at least one functional Inverted Terminal Repeat. The isolated recombinant nucleic acid molecule lacks overlapping sequences with the nucleic acid of the cell.Type: GrantFiled: July 30, 2001Date of Patent: August 31, 2004Assignee: Crucell Holland B.V.Inventors: Frits Jacobus Fallaux, Robert Cornelis Hoeben, Alex Jan van der Eb, Abraham Bout, Domenico Valerio
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Patent number: 6692966Abstract: The problem of replication-competent adenovirus in virus production is solved in that we have developed packaging cells that have no overlapping sequences with a new basic vector and thus, are suited for safe large scale production of recombinant adenoviruses. One of the additional problems associated with the use of recombinant adenovirus vectors is the host-defense reaction against treatment with adenovirus. Another aspect of the invention involves screening recombinant adenovirus vector lots, especially those intended for clinical use, for the presence of adenovirus E1 sequences, as this will reveal replication-competent adenovirus, as well as revertant E1 adenoviruses. It is also an aspect of the present invention to molecularly characterize the revertants that are generated in the newer helper/vector combinations.Type: GrantFiled: July 23, 2001Date of Patent: February 17, 2004Assignee: Crucell Holland B.V.Inventors: Frits J. Fallaux, Robert C. Hoeben, Abraham Bout, Domenico Valerio, Alex J. van der Eb
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Publication number: 20030166285Abstract: A method is provided for genetically modifying primate bone marrow cells, comprising isolating bone marrow cells from a primate and, by means which enhance the local concentration of retroviral particles, contacting the isolated bone marrow cells to cells that produce a recombinant amphotropic retrovirus with a genome based on a retroviral vector that contains the genetic information to be introduced into the bone marrow cells. Recombinant amphotropic retrovirus-producing cells, suitable for use in this method also are provided, as are genetically modified primate bone marrow cells with the capacity for regeneration in vivo.Type: ApplicationFiled: June 4, 2002Publication date: September 4, 2003Inventors: Domenico Valerio, Victor Willem Van Beusechem