Abstract: A laser system includes one or more harmonic generator blocks or elements including one or more crystals for converting a first wavelength (?1) laser beam into second, third and/or fourth wavelength (?2, ?3 and/or ?4) laser beams that may be output, with or without the first wavelength (?1) laser beam, on different beam paths. One or more of the first, second, third and/or fourth wavelength laser beams may travel or traverse in a crystal in one or multiple directions.

Abstract: The present invention relates to a method of producing a heterologous polypeptide of interest in a host cell, wherein the method comprises expressing a fusion protein comprising the heterologous polypeptide of interest and a fluorescent fusion partner in a host cell modified to include a nucleic acid encoding a lytic protein operably linked to a promoter, wherein translation of the lytic protein is under control of an RNA thermometer. Also provided are E. coli cells which express the fusion protein and include a nucleic acid encoding a lytic protein operably linked to a promoter, wherein translation of the lytic protein regulated by an RNA thermometer.

Abstract: The present invention relates to a bioreactor for the catalytic conversion of a substrate to a product using an immobilized enzyme. The immobilized enzyme is a histidine tagged enzyme, which binds to a nickel-nanoparticle coated cellulose matrix which is housed within the bioreactor. The invention also relates to methods of producing products by enzymatic catalysis using the bioreactor of the invention.