Abstract: Provided are separation systems and related methods for use in processing organic polymeric feed materials—such as plastics—to form pyrolysis oil. The disclosed systems can be operated in a continuous manner and utilize novel liquid-solid separation techniques integrated with a novel condensing approach so as to operate in a product-efficient and an energy-efficient manner.

Abstract: Provided are systems and related methods for processing organic polymeric feed materials—such as plastics—to form pyrolysis oil. The disclosed systems can be operated in a continuous manner and utilize novel liquid-solid separation techniques integrated with a novel condensing approach so as to operate in a product-efficient and an energy-efficient manner.

Abstract: An operations unit, comprising: a first chamber; a second chamber; a conduit extending through the first chamber and into the second chamber, the conduit being at least partially enclosed by a conduit jacket that defines an outer diameter, the conduit placing the second chamber into fluid communication with an environment exterior to the chamber, the second chamber comprising a wall facing the conduit jacket, and the second chamber being rotatable relative to the first chamber; a seal defining a boundary between the first chamber and the second chamber, the seal extending radially from the wall of the second chamber toward the conduit jacket, the seal comprising a flange, the flange defining an inner diameter, (a) the seal comprising a layered portion that comprises a plurality of ring-shaped portions, or (b) the seal comprising a brush that rotatably abuts the conduit jacket.

Abstract: Provided are separation systems and related methods for use in processing organic polymeric feed materials—such as plastics—to form pyrolysis oil. The disclosed systems can be operated in a continuous manner and utilize novel liquid-solid separation techniques integrated with a novel condensing approach so as to operate in a product-efficient and an energy-efficient manner.

Abstract: The present invention provides a method for lysing a fixed biological sample, wherein the fixed biological sample comprises crosslinks between nucleic acid molecules and protein molecules due to the fixation, said method comprising (a) lysing the fixed biological sample, wherein lysis involves digestion with a proteolytic enzyme; (b) heating the lysed sample to reverse crosslinks; (c) adding a proteolytic enzyme and performing a proteolytic digestion; optionally wherein one or more additional treatment steps are performed between step (b) and step (c). The provided nucleic acids are of high yield and quality and can be purified from the lysed sample.

Abstract: An operations unit, comprising: a first chamber; a second chamber; a conduit extending through the first chamber and into the second chamber, the conduit being at least partially enclosed by a conduit jacket that defines an outer diameter, the conduit placing the second chamber into fluid communication with an environment exterior to the chamber, the second chamber comprising a wall facing the conduit jacket, and the second chamber being rotatable relative to the first chamber; a seal defining a boundary between the first chamber and the second chamber, the seal extending radially from the wall of the second chamber toward the conduit j acket, the seal comprising a flange, the flange defining an inner diameter, (a) the seal comprising a layered portion that comprises a plurality of ring-shaped portions, or (b) the seal comprising a brush that rotatably abuts the conduit jacket.

Abstract: A thermal cracking treatment train, comprising: a rotatable kiln reactor; the rotatable kiln reactor being configured to receive a polymeric feed material, the reactor defining at least one interior wall that bounds an interior volume of the reactor, the interior volume defining an entrance and an exit along a direction of feed material travel, the rotatable kiln reactor comprising a section that comprises one or more sweeping features configured to sweep, with rotation of the kiln, a portion of the at least one interior wall, the kiln comprising a section that comprises one or more lifter features extending from the at least one interior wall and configured to, with rotation of the kiln, encourage material disposed on the one or more features to fall into the interior volume of the interior kiln; a combustor configured to provide a heated gas to the rotatable kiln reactor; and a devolatilization train.

Abstract: Provided are systems and related methods for processing organic polymeric feed materials—such as plastics—to form pyrolysis oil. The disclosed systems can be operated in a continuous manner and utilize novel liquid-solid separation techniques integrated with a novel condensing approach so as to operate in a product-efficient and an energy-efficient manner.

Abstract: The present invention relates to a composition for disrupting tissue material, the composition comprising solid disrupting particles in combination with at least one enzyme for enzymatic lysis and at least one chaotropic agent, as well as to a method for disrupting tissues material by simultaneously applying mechanical grinding or milling disruption and enzymatic digestion.

Abstract: A method for processing fibrous cellulosic material from algae comprising: (i) suspending the fibrous cellulosic material in water to form a suspension; and (ii) passing the suspension through at least one chamber having a large gap, at a high shear to produce cellulose nanofibrils. Also described are cellulose nanofibrils and cellulose nanocrystals, products, methods and uses of the same.

Abstract: The present invention pertains inter alia to a method for isolating poly(A) nucleic acids having a single stranded poly(A) stretch from a nucleic acid containing sample comprising: (a) providing a hybridization composition comprising: i) a nucleic acid containing sample; ii) a hybridization solution comprising: aa. a sodium salt; bb. a quaternary ammonium salt; wherein the components of the hybridization solution can be added as single solution to the sample or may be added separately in any order to the sample; iii) a capture probe capable of hybridizing to the poly(A) stretch of the poly(A) nucleic acids; and incubating said hybridization composition under conditions to form nucleic acid-hybrids between the poly(A) nucleic acids and the capture probe; (b) separating the formed hybrids from the remaining sample. The method is in particular suitable for efficiently isolating poly(A) RNA from various samples while avoiding carry-over of unwanted non-poly(A) nucleic acids such as rRNA.

Abstract: Provided is a method for isolating nucleic acids from a plant sample comprising (a) preparing a lysed sample wherein preparing comprises (i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and (ii) optionally clearing the lysate; (b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture; (c) obtaining a liquid phase from the mixture; and (d) isolating nucleic acids from the liquid phase. Also provided is a kit for use in such method.

Abstract: The present invention provides a lysis method for releasing microbial nucleic acids from microorganisms comprised in a plant sample, comprising mechanically disrupting the plant sample in a liquid lysis composition using at least two types of solid disrupting particles, wherein (i) the first type is provided by one or more disrupting particles having a size of at least 1.5 mm and (ii) the second type is provided by a plurality of disrupting particles having a size of 1 mm or less. Also provides is a method for isolating nucleic acids including microbial nucleic acids from a plant sample and kits.

Abstract: The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

Abstract: The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.