Abstract: The invention relates to a transgenic plant overexpressing a polynucleotide encoding a plant GTP cyclohydrolase I (GTPCHI) enzyme. The present invention also relates to a product comprising the transgenic plant of the invention or a harvestable part, plant organ, plant tissue, plant cell, seed, or a propagule of said plant. The present invention further elucidate the use of the plant of the invention to cross breed with other plants, as biofortification to enrich the quality of the food intake of an animal or human subject, for the manufacture of a medicament to prevent folate insufficiency, or, for the treatment of folate insufficiency or diseases or disorders resulting from said insufficiency in an animal or human subject.

Abstract: Disclosed is substantially pure DNA encoding a crucifer ACC synthase polypeptide; a promoter functional in immature plant tissues which is capable of ethylene induction; and methods of using such promoters to express recombinant proteins or RNA and to regulate ethylene-inducible events of a plant, e.g., fruit ripening or senescence, especially during early stages of plant development.