Abstract: A collecting system is provided that can include a probe configured to collect pathogens from a surrounding fluid, an elution chamber containing a liquid solvent and configured to receive the probe to elute the pathogens collected on the probe using the liquid solvent, and a heater configured to lyse the pathogens to release the genetic material of the pathogens into the liquid solvent.

Abstract: Provided herein are feedback controller circuits and cell state classifiers for tunable phosphorylation-based transcriptional regulation of gene expression in cells based on intracellular miRNA profiles and degradation by small molecules. Also provided are methods of using feedback controller circuits and cell state classifiers for determining the cell state, and methods of treating cells and subjects using feedback controller circuits and cell state classifiers encoding therapeutic output molecules.

Abstract: The present disclosure, at least in part, relates to an engineered incoherent feed forward loop (iFFL) comprising a first transcription unit encoding an endoribonuclease and a second transcription unit encoding an output molecule and an endoribonuclease target site located in the 5? UTR of the output molecule coding sequence. The engineered iFFL, at least in part, can be used for sustained expression of an output molecule despite of transcription resource disturbance in a given environment.

Abstract: The present disclosure, at least in part, relates to an engineered incoherent feed forward loop (iFFL) comprising a first transcription unit encoding an endoribonuclease and a second transcription unit encoding an output molecule and an endoribonuclease target site located in the 5? UTR of the output molecule coding sequence. The engineered iFFL, at least in part, can be used for sustained expression of an output molecule despite of transcription resource disturbance in a given environment.

Abstract: A back-propagating intersection collision avoidance system is provided. The system can include a first vehicle and a second vehicle, the first and second vehicles each operable to approach an intersection at a definable velocity and acceleration. In addition, the intersection can have a collision zone in which the first and second vehicles will collide if they are present there at the same time. The first vehicle can have a processing unit with a controller and a microprocessor, the microprocessor having an algorithm with a disturbance model. The processing unit is operable to back-propagate from the collision zone a capture set as a function of a disturbance for the first and second vehicles.

Abstract: The present invention discloses a back-propagating intersection collision avoidance (ICA) system for preventing two or more vehicles from colliding at an intersection. The ICA system can calculate predicted positions of the two or more vehicles in the near future, and both the current and future positions can be broadcast to surrounding vehicles using vehicle-to-vehicle communication. For each vehicle, a set of states, for example position, speed, acceleration, and the like, where a collision is imminent can be identified using state information for a local vehicle, a remote vehicle, and a known collision zone for the intersection. If the current states of the vehicles are determined to be in danger of entering the collision zone, the ICA system can control the vehicles to perform evasive driving maneuvers and/or alert the drivers.

Abstract: A back-propagating intersection collision avoidance system is provided. The system can include a first vehicle and a second vehicle, the first and second vehicles each operable to approach an intersection at a definable velocity and acceleration. In addition, the intersection can have a collision zone in which the first and second vehicles will collide if they are present there at the same time. The first vehicle can have a processing unit with a controller and a microprocessor, the microprocessor having an algorithm with a disturbance model. The processing unit is operable to back-propagate from the collision zone a capture set as a function of a disturbance for the first and second vehicles.

Abstract: The present invention discloses a back-propagating intersection collision avoidance (ICA) system for preventing two or more vehicles from colliding at an intersection. The ICA system can calculate predicted positions of the two or more vehicles in the near future, and both the current and future positions can be broadcast to surrounding vehicles using vehicle-to-vehicle communication. For each vehicle, a set of states, for example position, speed, acceleration, and the like, where a collision is imminent can be identified using state information for a local vehicle, a remote vehicle, and a known collision zone for the intersection. If the current states of the vehicles are determined to be in danger of entering the collision zone, the ICA system can control the vehicles to perform evasive driving maneuvers and/or alert the drivers.