Abstract: Certain embodiments here in are directed to a method of treating a tissue associated with a defect in a human including wrinkles, rhytids, depressed scar, cutaneous depressions, stretch marks, hyperplasia of the lip, nasolabial fold, melolabial fold, scarring from acne vulgaris, and post-rhinoplasty irregularity. The tissue defect may be treated by introducing a plurality of in vitro cultured autologous fibroblast cells at or proximal to the defect area of the patient's tissue. The autologous fibroblast cells may have been cultured in vitro to expand the number of fibroblast cells in at least one medium that comprises autologous serum. The autologous fibroblast cell cultures may be derived from connective tissue, dermal, fascial fibroblasts, papillary fibroblasts, and/or reticular fibroblasts.

Abstract: The clean room, which exceeds the Class 100 standards, constructed out of structural materials that are free of metal, in particular aluminum. The room is sterile and allows for a variety of manipulations to be performed including sensitive analysis steps. Intrinsic aluminum and most metal contamination is eliminated by constructing the clean room with aluminum free structural materials. Extrinsic contamination is prevented by following standard clean room procedures in a two-door positive pressure entry system under a single self-motorized ULPA filtered air flow. Analysis of samples is conducted under a vertical laminar ULPA filtered air flow at at least a Class 10 limit particle concentration standard. In addition, a clean room containment center wherein samples are prepared and processed inside a modular containment center that is under a negative pressure allowing a horizontal laminar ULPA filtered air flow that provides a Class 1 limit particle concentration rating.