Abstract: Invention is a formed plate or sheet product which attaches to the underside of a vehicle, operating in shear with some bending. The formed plate or sheet product connects the Front Suspension Assembly, the Rear Suspension Assembly, and the Structural Body of a vehicle of unitary construction or frame-on-body construction or any combination thereof. The purpose the invention is to a) increase torsional rigidity of a vehicle and b) reduce torsional and translational compliance between the front suspension assembly, the rear suspension assembly, and the structural body. The invention forms a monolithic component, attaching to the vehicle using one or more of the auto manufacturer's mounting points.

Abstract: Disclosed is a binding assay for proteases and phosphatases, which contain cysteine in their binding sites or as a necessary structural component for enzymatic binding. The sulfhydryl group of cysteine is the nucleophilic group in the enzyme's mechanistic proteolytic and hydrolytic properties. The assay can be used to determine the ability of new, unknown ligands and mixtures of compounds to competitively bind with the enzyme versus a known binding agent for the enzyme, e.g., a known enzyme inhibitor. By the use of a mutant form of the natural or native wild-type enzyme, in which serine, or another amino acid, e.g., alanine, replaces cysteine, the problem of interference from extraneous oxidizing and alkylating agents in the assay procedure is overcome. The interference arises because of oxidation or alkylation of the sulfhydryl, --SH (or --S.sup.-), in the cysteine, which then adversely affects the binding ability of the enzyme.