Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilised by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilized by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilized by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilized by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilised by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilised by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilised by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilised by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: Ingenol angelate is a potent anticancer agent, and can be stabilised by dissolving it in an aprotic solvent in the presence of an acidic buffer.

Abstract: The present invention relates to a composition for use as an antimicrobial medicament comprising a biocidally active compound. The invention also relates to a medicament comprising at least one biocidally active compound and a fungal cell or fungal cell fragment wherein molecules of the at least one biocidally active compound are encapsulated or partially encapsulated by the fungal cell or fungal cell fragment.

Abstract: The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is ruthenium (III) pentaamine pyridine and the polynucleotide that is detected is DNA. Further, techniques for enhancing detectable contrast between hybridized and unhybridized nucleic acids are disclosed. In particular, the use of elongated target strands as well as the use of uncharged probe strands are discussed.

Abstract: A universal tag assay is disclosed wherein at least one invasive cleavage reaction (ICR) is used to generate tagged molecules having identifier tags corresponding to target nucleotide sequences, and further wherein hybridization of any tagged molecule with a complementary detection probe on a universal detector indicates the presence of the corresponding target in the sample being assayed. Preferred embodiments include the use of ICR to generate molecules suitable for use in the universal tag assay to detect variant nucleotide sequences including single nucleotide polymorphisms (SNPs), allelic variants, and splice variants. Hybridization of tagged molecules to detection probes is preferably detected by electrochemical readout, in particular the use of ruthenium amperometry to detect hybridization of identifier tags to detection probes immobilized on a universal detector, preferably a universal chip having gold or carbon electrodes.

Abstract: The present application provides methods and compositions for use in detecting the presence of a target nucleic acid in a sample. In some embodiments, the methods employ sequestering agents which specifically interact with complementary nucleic acids which will be ligated together if the target nucleic acid is present in the sample. Detection of a ligation product comprising the complementary nucleic acids indicates that the target nucleic acid is present in the sample.

Abstract: The present disclosure relates to methods of detecting nucleic acid hybridization by monitoring an output signal. Some advantageous embodiments include techniques which can augment the signal. One such technique involves catalytic detection, such as with a peroxidase or other enzyme. Another technique involves “on-chip” amplification to enlarge the nucleic acid after hybridization has occurred.

Abstract: Disclosed herein are methods of destabilizing double-stranded nucleic acid hybridization using an enzyme comprising DNA N-glycosylase activity. Also disclosed herein is the detection of a double-stranded target DNA wherein the hybridization of duplex strands has been at least partially disrupted thereby permitting invasion of a probe strand. Also disclosed herein are methods of using an enzyme comprising DNA N-glycosylase activity to generate single-stranded circular nucleic acids.

Abstract: The present disclosure relates to methods for amplifying nucleic acids and methods for detecting the amplified nucleic acid products. Nucleic acid amplification and detection can occur in solution or on a solid support.

Abstract: The present disclosure relates to methods for generating single-stranded DNA molecules of defined sequence and length. Specifically, a region of template containing target sequence is amplified by PCR or RCA, exogenous sequence is introduced by primers or probes used in amplification, double-stranded amplification products are converted to single-stranded amplification products, and single-stranded amplification products are trimmed to produce short single-stranded DNA molecules of defined sequence and length.