Abstract: Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Abstract: Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Abstract: Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Abstract: In a phototypesetter, an array of light emitting diodes is energized in synchronization with an associated staggered array of fiber optic filaments. The array of filaments is slanted relative to the direction of movement, and type may be set in both forward and reverse directions. Type font data is stored in a compressed form in a first microprocessor subsystem. That compressed data is expanded in real time for each character in a high speed microprocessor subsystem. Character image data in the form of character contours is written into alternating RAM groups. The contour data is then read from the RAM groups on a slice basis and color data is generated. The alternate RAM groups allow for proper coloring of overlapping character images. The expanded data is then handled by a logic unit to allow for the staggered fiber optic filaments and for the slant of the fiber optic filament head in both forward and reverse directions.

Abstract: In a flexible, fiber optic belt 16 for the coherent transmission of light from an LED light source 22 a planar array of fiber optic filaments 34 extend along the length of the belt 16 and are positioned by transverse elements 79 along the major neutral plane of the belt. The fiber optic belt 16 is restrained to movement only in the direction normal to the major neutral plane by means of one or more rigid flat strips 25 parallel to the major neutral plane and the light source 22 is thermoelectrically cooled to improve the availability of light supplied by the diodes. In a method for producing the fiber optic cable, transverse positioning elements 79 are layered on a cable mold 56, one or more fiber optic filaments 34 are placed upon the transverse positioning elements 79. Fiber optic filaments 34 are pressed onto an adhesive layer adjacent to the LED's 48 and bonded by the melting of the adhesive.

Abstract: A novel device for automatically deflating a blood pressure cuff with a standard two way valve is disclosed. An adjustable deflation rate consistent with normally accepted medical practice of two or three millimeters per heartbeat may be maintained. A two state valve and associated control circuitry for maintaining a constant deflation rate are described. This allows accurate sensing of Korotkoff sounds at different pressure levels.