Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest in a sample, comprising the steps of: (a) reacting the sample containing the target sequence of interest with a first nucleic acid probe, so as to cause hybridization between complementary portions of the target and the probe, wherein the first probe comprises 5′ and 3′ portions complementary to respective, substantially adjacent portions of the target sequence and an intervening non-complementary portion which does not become hybridized to the target, thereby creating a loop region looped out from a complex formed between the first probe and the target, such that non-contiguous portions of the first probe are brought into close proximity; (b) hybridizing a second nucleic acid probe to the non-contiguous portions of the first probe, (c) initiating nucleic acid synthesis, using the first probe as template, in a manner dependent upon hybridization of the second probe to the first probe; and (d) detecting th

Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of:

Abstract: Disclosed is a method of detecting a nucleic acid sequence of interest in a sample, the method comprising contacting the sample with first and second probes; the first probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest; the second probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest but complementary to that portion of the first probe which is non-complementary to the sequence of interest, such that the first and second probes are capable of hybridising to the sequence of interest in an adjacent or substantially adjacent manner, so as to allow complementary portions of the first and second probes to hybridize to each other; causing extension of the first probe with a nucleic acid polymerase, using the second probe as a template; and detecting directly or indirectly the extension of

Abstract: Disclosed is a method of detecting the presence of a nucleic acid target sequence of interest, the method comprising the steps of: (a) adding first and second nucleic acid probes to a sample comprising the sequence of interest, so as to form a complex comprising three strands of nucleic acid, wherein the first probe comprises the full length sequence of a first strand of a double stranded promoter, the target sequence comprises an end part of a second strand of the double stranded promoter which is complementary to a part of the first strand, and the second probe comprises the rest of the second strand of the double stranded promoter which is complementary to a part of the first strand, such that a functional promoter is formed when the first probe is hybridized to both the target sequence and to the second probe; (b) adding a polymerase which recognizes the promoter, so as to cause the de novo synthesis of nucleic acid from the promoter present in the complex; and (c) detecting directly or indirectly the