Abstract: There is provided an apparatus and method for collection and filtration of a sample having a sample holder, a filtrate holder and a connection conduit having a sample receiving end and a filtrate receiving end and containing a filter. The sample holder has an entry end portion and an exit end portion, with a diaphragm normally closing the exit end portion. The filtrate holder has a closeable receiving end portion for receiving the filtered sample. The connection conduit has a rupturing device for rupturing the diaphragm on exit end portion of the sample holder. When the sample holder is inserted into the connection conduit, the diaphragm rupturing device ruptures the diaphragm, allowing the sample be filtered by the filter located within the connection conduit and the filtrate is collected in the filtrate holder. Also described is a method of collecting a biological sample, and filtering the sample, and holding the filtrate in a filtrate holder.