Abstract: The present invention relates to the asthma-protective effects of farm dust, specifically to a composition comprising barn dust extract including isolated fractions of an Amish barn dust extract comprising different bioactive components that have an ability to protect against asthma. In particular, the present invention describes a barn dust composition with asthma-protective properties, the barn dust composition comprising one or more bioactive fractions extracted from barn dust, said one or more bioactive fractions comprising one or more proteins and one or more fatty acids. The one or more bioactive fractions of the barn dust compositions may comprise molecular weights of 30-100 kDa, particularly 28-64 kDA, that include eight (8) target proteins, which have potential to proactively prevent the induction of asthma and to treat current cases of asthma. The present invention also relates to an in vitro method for screening allergic compounds.

Abstract: The present invention relates to novel methods and routes of administration of specific bacterial extracts obtainable by alkaline lysis of Gram positive or Gram negative bacterial species and delivery devices for treating and/or preventing asthma, asthma exacerbation, allergic asthma conditions and/or wheezing associated symptoms and/or associated with microbiota related to respiratory disorders in a subject in need thereof comprising administering a therapeutically effective amount of bacterial extract obtainable from Gram positive or Gram negative bacterial species wherein said bacterial extract is obtainable by alkaline lysis.

Abstract: The present invention relates to a method of preparing a barn dust extract, said barn dust extract, and a composition comprising said barn dust extract. The barn dust extract is useful in the prevention or treatment of a disease.