Abstract: Provided are a composition and a kit for diagnosing bladder cancer, breast cancer, cervical cancer, colorectal cancer, gastric cancer, liver cancer, lung cancer, ovarian cancer, pancreatic cancer, prostate cancer, and renal cancer, and a method for diagnosing cancer or a method for detecting a biomarker for diagnosing cancer, using the same. The present invention may be used as a composition or a kit for point-of-care testing-type diagnosis, and may diagnose bladder cancer, breast cancer, cervical cancer, colorectal cancer, gastric cancer, liver cancer, lung cancer, ovarian cancer, pancreatic cancer, prostate cancer, and renal cancer with high accuracy, sensitivity, and specificity.

Abstract: Maintenance of adult tissues depends on stem cell self-renewal in local niches. Spermatogonial stem cells (SSC) are germline adult stem cells necessary for spermatogenesis and fertility. The present invention relates utilization of testicular endothelial cells (TECs) in the SSC niche producing glial cell line-derived neurotrophic factor (GDNF) and other factors to support human and mammal SSCs in long-term culture. The present invention also relates to utilization of five factors sufficient for long-term maintenance of human and mammal SSC colonies in feeder-free cultures. Male cancer survivors after chemotherapy are often infertile since SSCs are highly susceptible to cytotoxic injury. Transplantation of TECs alone is used to restore spermatogenesis in mice after chemotherapy-induced depletion of SSCs.