Abstract: The present invention relates to a marker composition for pregnancy diagnosis in cattle, a pregnancy diagnosis composition, and a pregnancy diagnosis method, which use a pregnancy-specific protein in cattle. The present inventors discovered an ?1G subunit protein of the T-type calcium channel expressed specifically in pregnant cows, and produced a specific antibody against this protein. Therefore, the present invention has the effect of detecting pregnancy in cows easily, quickly, and accurately early in the pregnancy.

Abstract: The present invention relates to a marker composition for pregnancy diagnosis in cattle, a pregnancy diagnosis composition, and a pregnancy diagnosis method, which use a pregnancy-specific protein in cattle. The present inventors discovered an ?1G subunit protein of the T-type calcium channel expressed specifically in pregnant cows, and produced a specific antibody against this protein. Therefore, the present invention has the effect of detecting pregnancy in cows easily, quickly, and accurately early in the pregnancy.

Abstract: Disclosed is a method of analyzing a protein, comprising the steps of: (A) labeling small amounts of protein samples with CyDyes having different fluorescent properties, respectively; (B) mixing 50-100 ?g of each of the protein samples, labeled in step (A), with 1-5 mg of each of one or more of the unlabeled protein samples, and subjecting the mixture to electrophoresis; (C) subjecting the electrophoresed gel to fluorescence analysis to detect a difference in protein distribution between the protein samples; and (D) excising spot(s), showing the difference in protein distribution, from the gel, and isolating and identifying from the spots a protein, which is different in protein distribution between the protein samples. According to the present invention, a protein from a gel can be identified by MALDI-TOF, while simultaneously analyzing the gel qualitatively and quantitatively using a difference in the fluorescence of CyDyes with very high sensitivity.