Abstract: A preparation method of glassless grain-oriented silicon steel includes the following operations. During a decarburization annealing, a thickness of an oxide film on a surface of strip is 1.5-2.5 ?m; an atomic weight ratio of Si element and Fe element in the oxide film satisfies: Si/(Si+Fe)?0.76; during a high-temperature annealing, a cooling stage includes sequentially: cooling with an inner cover when a temperature drops from 1200° C. to 500° C.; wherein a protective gas is a mixed gas containing nitrogen and hydrogen, and a volume percentage of the hydrogen in the mixed gas is >3%; cooling with the inner cover when the temperature drops from 500° C. to 200° C.; wherein the protective gas is nitrogen; and cooling in air by removing the inner cover when the temperature is <200° C.