Abstract: The disclosure relates to the technical field of prosthodontics, and in particular, to a method and system for digital fabrication of a denture. The method includes: editing attributes of a three-dimensional model of a prefabricated tooth, and establishing a template database; obtaining oral data of a patient, and performing tooth arrangement design and base design; determining, based on a virtual denture model, a three-dimensional model of a positioning auxiliary body; fabricating a positioning auxiliary body according to the three-dimensional model of the positioning auxiliary body; and using the positioning auxiliary body to physically position and arrange the physical prefabricated tooth corresponding to the three-dimensional model of the prefabricated tooth, and bonding the physical prefabricated tooth to a gingival base material, to form the denture.