Abstract: A high-throughput automated gene synthesis device based on a cluster array includes a substrate and a microwell plate; the substrate is provided with a plurality of clusters of micropores; the inner wall surface of the micropores is chemically modified as a solid phase carrier for nucleic acid synthesis, or the micropore is filled with solid phase carriers; the clusters of micropores are arranged in a cluster array and each cluster of micropores has the same size and corresponding position as each well on the microwell plate. When using the device to synthesize oligonucleotides, by automatically recovering the synthesized oligonucleotides into a standard SBS plate of the corresponding size under the device, the oligonucleotide pool for each gene is formed. The yield of oligonucleotides is in picomole level, which is used for subsequent polymerase-mediated gene assembly (PCA) or ligase-mediated gene assembly (LCR) without amplification.

Abstract: The present invention relates to a method and apparatus of manufacturing nickel-metal-hydride alloy powder material. The furnace charge of nickel-metal-hydride alloy is melted in vacuum or argon atmosphere in this invention. After melting, the molten alloy is gas atomized to fine spherical powder or centrifugal atomized to flaky shape. Then the powders are fed into a hydrogen heat treatment chamber for hydrogen heat treatment and pulverization. This invention integrates the melting, pulverizing and hydrogen treatment of nickel-metal hydride alloy powder into a whole step. It can charge and pulverize continuously and is suitable for the large-scale industrialized production of homogeneous composition and least segregation nickel-metal hydride alloy powder.