Abstract: An optimization method for capturing proteins by multi-column continuous chromatography (MCC), including the following steps: step 1, under the conditions of a set loading protein concentration and an arbitrary load residence time, performing a single time of protein breakthrough experiment to obtain a protein breakthrough curve; step 2, under a set breakthrough percentage for a target protein, integrating the breakthrough curve to obtain a single-column loading capacity and establishing a linear relationship between the interconnected load time and the load residence time; step 3, solving for the optimal number of operating columns for capturing proteins by MCC based on step 2; step 4, solving for the optimal load residence time for capturing proteins by MCC based on step 2, step 3; and step 5, solving for the maximum productivity of capturing proteins by MCC based on step 4.

Abstract: An optimization method for capturing proteins by multi-column continuous chromatography (MCC), including the following steps: step 1, under the conditions of a set loading protein concentration and an arbitrary load residence time, performing a single time of protein breakthrough experiment to obtain a protein breakthrough curve; step 2, under a set breakthrough percentage for a target protein, integrating the breakthrough curve to obtain a single-column loading capacity and establishing a linear relationship between the interconnected load time and the load residence time; step 3, solving for the optimal number of operating columns for capturing proteins by MCC based on step 2; step 4, solving for the optimal load residence time for capturing proteins by MCC based on step 2, step 3; and step 5, solving for the maximum productivity of capturing proteins by MCC based on step 4.

Abstract: This invention relates to a hybrid ligand, a hybrid biomimetic chromedia and a preparing method and a use thereof, wherein the hybrid biomimetic chromedia takes hydrophilic porous microsphere as a substrate in chromatography, activated with allyl bromide and undergoing bromo-alcoholization with N-bromosuccinimide, then coupled with the hybrid ligands. The sequence of the hybrid ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole. The hybrid biomimetic chromedia has both of the two functional groups of phenylalanine-tyrosine-glutamine tripeptide and aminobenzimidazole, while maintaining the high antibody selectivity of polypeptide ligand, hydrophobic electric charge inductive ligand is introduced to achieve more moderate elution requirement, realizing effective antibody separation.

Abstract: This invention relates to a hybrid ligand, a hybrid biomimetic chromedia and a preparing method and a use thereof, wherein the hybrid biomimetic chromedia takes hydrophilic porous microsphere as a substrate in chromatography, activated with allyl bromide and undergoing bromo-alcoholization with N-bromosuccinimide, then coupled with the hybrid ligands. The sequence of the hybrid ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole. The hybrid biomimetic chromedia has both of the two functional groups of phenylalanine-tyrosine-glutamine tripeptide and aminobenzimidazole, while maintaining the high antibody selectivity of polypeptide ligand, hydrophobic electric charge inductive ligand is introduced to achieve more moderate elution requirement, realizing effective antibody separation.