Patents by Inventor Dongxin Zhao

Dongxin Zhao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Efficient genetic screening method
    Patent number: 11913017
    Abstract: A guide RNA comprising: a gRNA spacer sequence at the 5? end of the guide RNA, wherein the spacer sequence is complementary to a target gene, a scaffold sequence that binds to Cas9, and an RNA capture and sequencing domain comprising: a barcode sequence, and a primer binding sequence; nucleic acids and vectors encoding the guide RNA; cells expressing the guide RNA; and a library comprising a plurality of guide RNAs. Also disclosed are methods of introducing a genetic perturbation into a cell, methods of assessing an effect of at least one genetic perturbation on RNA expression in a cell, methods of identifying nucleic acid sequences associated with a disease state and a method of identifying candidate therapeutic agents.
    Type: Grant
    Filed: June 28, 2017
    Date of Patent: February 27, 2024
    Assignee: The Regents of the University of California
    Inventors: Kun Zhang, Prashant Mali, Yan Wu, Dongxin Zhao
  • EFFICIENT GENETIC SCREENING METHOD
    Publication number: 20190330661
    Abstract: A guide RNA comprising: a gRNA spacer sequence at the 5? end of the guide RNA, wherein the spacer sequence is complementary to a target gene, a scaffold sequence that binds to Cas9, and an RNA capture and sequencing domain comprising: a barcode sequence, and a primer binding sequence; nucleic acids and vectors encoding the guide RNA; cells expressing the guide RNA; and a library comprising a plurality of guide RNAs. Also disclosed are methods of introducing a genetic perturbation into a cell, methods of assessing an effect of at least one genetic perturbation on RNA expression in a cell, methods of identifying nucleic acid sequences associated with a disease state and a method of identifying candidate therapeutic agents.
    Type: Application
    Filed: June 28, 2017
    Publication date: October 31, 2019
    Applicant: The Regents of the University of California
    Inventors: Kun ZHANG, Prashant MALI, Yan WU, Dongxin ZHAO
  • METHODS FOR OBTAINING HEPATOCYTES, HEPATIC ENDODERM CELLS AND HEPATIC PROGENITOR CELLS BY INDUCED DIFFERENTIATION
    Publication number: 20120190059
    Abstract: The present invention discloses a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells (iPS cells) into hepatocytes, a method for inducing the differentiation of embryonic stem cells or induced pluripotent stem cells into hepatic endoderm cells, and a method for inducing the differentiation of embryonic stem cells (ESC) or induced pluripotent stem cells into hepatic progenitor cells. The present invention also provides the hepatocytes, hepatic endoderm cells and hepatic progenitor cells obtained by above methods, and the uses of these cells.
    Type: Application
    Filed: July 23, 2010
    Publication date: July 26, 2012
    Applicant: BEIJING HUAYUANBOCHUANG TECHNOLOGY CO., LTD.
    Inventors: Hongkui Deng, Mingxiao Ding, Dongxin Zhao, Song Chen, Zhihua Song