Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.

Abstract: A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.

Abstract: A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.

Abstract: A method for identifying a quantitative trait locus associated with desirable nutritional traits in canola includes: analyzing a population of canola plants or germplasm for desirable nutritional traits; determining the genotype of the canola plants or germplasm using at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111; mapping the canola plants or germplasm for the presence of a quantitative trait locus (QTL) associated with the markers; and associating the QTL with the desirable nutritional trait. An isolated and/or recombinant nucleic acid includes a sequence associated with a quantitative trait locus (QTL), wherein the QTL is associated with a desirable nutritional trait in a canola plant or germplasm and wherein the QTL is further associated with at least one marker selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:111.

Abstract: The present invention relates to a new and distinctive canola, designated G5428584H. Also included are seeds of canola G5428584H, to the plants, or plant parts, of canola G5428584H and to methods for producing a canola plant produced by crossing the canola G5428584H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G5428584H.

Abstract: The present invention relates to a new and distinctive canola, designated G146645A/B. Also included are seeds of canola G146645A/B, to the plants, or plant parts, of canola G146645A/B and to methods for producing a canola plant produced by crossing the canola G146645A/B with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G146645A/B.

Abstract: The present invention relates to a new and distinctive canola, designated G263532R. Also included are seeds of canola G263532R, to the plants, or plant parts, of canola G263532R and to methods for producing a canola plant produced by crossing the canola G263532R with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G263532R.

Abstract: The present invention relates to a new and distinctive canola, designated G3697124H. Also included are seeds of canola G3697124H, to the plants, or plant parts, of canola G3697124H and to methods for producing a canola plant produced by crossing the canola G3697124H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G3697124H.

Abstract: The present invention relates to a new and distinctive canola, designated G1992650A. Also included are seeds of canola G1992650A, to the plants, or plant parts, of canola G1992650A and to methods for producing a canola plant produced by crossing the canola G1992650A with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G1992650A.

Abstract: The present invention relates to a new and distinctive canola, designated G1562823A. Also included are seeds of canola G1562823A, to the plants, or plant parts, of canola G1562823A and to methods for producing a canola plant produced by crossing the canola G1562823A with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G1562823A.

Abstract: The present invention relates to a new and distinctive canola, designated G6667223H. Also included are seeds of canola G6667223H, to the plants, or plant parts, of canola G6667223H and to methods for producing a canola plant produced by crossing the canola G6667223H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G6667223H.

Abstract: The present invention relates to a new and distinctive canola, designated G6667223H. Also included are seeds of canola G6667223H, to the plants, or plant parts, of canola G6667223H and to methods for producing a canola plant produced by crossing the canola G6667223H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G6667223H.

Abstract: The present invention relates to a new and distinctive canola, designated G30853A. Also included are seeds of canola G30853A, to the plants, or plant parts, of canola G30853A and to methods for producing a canola plant produced by crossing the canola G30853A with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G30853A.

Abstract: The present invention relates to a new and distinctive canola, designated G1934899R. Also included are seeds of canola G1934899R, to the plants, or plant parts, of canola G1934899R and to methods for producing a canola plant produced by crossing the canola G1934899R with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G1934899R.

Abstract: The present invention relates to a new and distinctive canola, designated G2537376H. Also included are seeds of canola G2537376H, to the plants, or plant parts, of canola G2537376H and to methods for producing a canola plant produced by crossing the canola G2537376H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G2537376H.

Abstract: The present invention relates to a new and distinctive canola, designated G175274R. Also included are seeds of canola G175274R, to the plants, or plant parts, of canola G175274R and to methods for producing a canola plant produced by crossing the canola G175274R with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G175274R.

Abstract: The present invention relates to a new and distinctive canola, designated G263532R. Also included are seeds of canola G263532R, to the plants, or plant parts, of canola G263532R and to methods for producing a canola plant produced by crossing the canola G263532R with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G263532R.

Abstract: The present invention relates to a new and distinctive canola, designated G5428584H. Also included are seeds of canola G5428584H, to the plants, or plant parts, of canola G5428584H and to methods for producing a canola plant produced by crossing the canola G5428584H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G5428584H.

Abstract: The present invention relates to a new and distinctive canola, designated G146645A/B. Also included are seeds of canola G146645A/B, to the plants, or plant parts, of canola G146645A/B and to methods for producing a canola plant produced by crossing the canola G146645A/B with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G146645A/B.

Abstract: The present invention relates to a new and distinctive canola, designated G2537376H. Also included are seeds of canola G2537376H, to the plants, or plant parts, of canola G2537376H and to methods for producing a canola plant produced by crossing the canola G2537376H with itself or another canola genotype, and the creation of variants by mutagenesis or transformation of canola G2537376H.