Patents by Inventor Donna Paddock

Donna Paddock has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for refolding enzymes
    Patent number: 7732183
    Abstract: The invention provides methods for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. In various aspects, the method includes the steps of expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, diluting the solubilized BACE polypeptide in an aqueous solution at a temperature of about 1° C. to 15° C., and incubating the diluted sample at a temperature of about 4° C. to 15° C. until the recombinant BACE polypeptide folds into an active enzyme.
    Type: Grant
    Filed: February 7, 2007
    Date of Patent: June 8, 2010
    Assignee: Elan Pharmaceuticals, Inc.
    Inventors: Alfredo Tomasselli, Robert Heinrikson, Donna Paddock, Ana Mildner, Thomas Emmons
  • Method for refolding enzymes
    Patent number: 7186539
    Abstract: The invention provides methods for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. In various aspects, the method includes the steps of expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, diluting the solubilized BACE polypeptide in an aqueous solution at a temperature of about 1° C. to 15° C., and incubating the diluted sample at a temperature of about 4° C. to 15° C. until the recombinant BACE polypeptide folds into an active enzyme.
    Type: Grant
    Filed: August 29, 2002
    Date of Patent: March 6, 2007
    Assignee: Pharmacia & UpJohn Company
    Inventors: Alfredo Tomasselli, Robert Heinrikson, Donna Paddock, Ana Mildner, Thomas Emmons