Abstract: Improvements in the efficiency and sensitivity of restriction fragment length polymorphism (RFLP) detection in target sequences are realized by developing primers that are specific for nucleic acid sequences, labelling those primers with distinguishable, non-radioactive labels, such as chromophores, and applying the primers in sets such that, after amplification of the target sequence, specific sequences, in particular those of RFLPs, can be identified by virtue of the probe(s) which hybridized.