Abstract: A method of extracting human progenitor cells from perivascular tissue of human umbilical cord. The extracted cells are then co-cultured with nonhematopoietic stem cells and are useful to grow and repair human tissues including bone. Also included are related methods and compositions related thereto.

Abstract: The disclosure describes a method for producing native mammalian biological molecules by electromagnetically stimulating mammalian cells using an electromagnetic field. The mammalian cells can be grown in a chamber that is exposed to a time varying electromagnetic force. The biological molecules can be isolated from the cells.

Abstract: A bioreactor, kit, a method of using the device to promoting growing and/or culture of a cell, and a method for regenerating and/or improving the function of mammalian cells. The bioreactor includes a controlling circuit coupled to magnetic field emitter that emits relatively steep and short-lived magnetic field pulses during these an active ephemeral period. The bioreactor also provides a relatively long-term inactive phase in which no magnetic field pulses are imposed. The kit includes the unassembled components of the bioreactor. The method of using the bioreactor and of regenerating and/or improving the function of mammalian cells includes the step of applying a time variant magnetic field through the cell to promote growing and/or culturing, and then introducing the cells to a mammal to regenerate cells.

Abstract: An osteo or tissue healing kit and method of promoting the healing of compromised bone or tissue in a living mammal. The osteo or tissue healing device of the kit includes a magnetic field emitter and a controlling circuit, and at least one stem cell. The method of using the osteo or tissue healing kit includes placing at least one stem cell within the compromised bone or tissue and applying a time variant magnetic field.

Abstract: A composition is disclosed with the composition comprising a mixture of natively glycosylated mammalian biological molecules produced by electromagnetically stimulating living mammalian cells.

Abstract: An osteo or tissue healing device, kit and method of using the device to promote healing of compromised bone or tissue in a living mammal is disclosed. The osteo or tissue healing device includes a magnetic field emitter and a controlling circuit. The magnetic field emitter of the device is electrically coupled to a controlling circuit in which the magnetic field emitter is configured to provide a time variant magnetic field when driven by an electric pulse train from the controlling circuit in such a manner that the time variant magnetic field results in a magnetic (B) field exhibiting a magnetic slew rate of at least about 10 kiloGauss/sec. The controlling circuit of the device is electrically coupled to the magnetic field emitter and is configured to be powered by a power source, in which the controlling circuit is configured to output the electric pulse train driving the magnetic field emitter.

Abstract: A method of extracting human progenitor cells from perivascular tissue of human umbilical cord. The extracted cells are then co-cultured with hematopoetic stem cells and are useful to grow and repair human tissues including bone. Also included are related methods and compositions related thereto.

Abstract: The present invention is directed to the expansion, preferably TVEMF-expansion, of mammalian blood adult stem cells, wherein the expansion takes place in a rotating bioreactor, preferably a TVEMF-bioreactor, to compositions resulting from the expanded cells, and to a method of treating an epithelial cell/tissue related disease or condition or repairing tissue of skin, mouth or ear with the compositions.

Abstract: The present invention is directed to the process of preparing an expanded peripheral blood stem cell composition, preparing a TVEMF-expanded peripheral blood stem cell composition, methods of producing the same, and to repair of tissue and/or function with expanded, and TVEMF-expanded, peripheral blood stem cells.

Abstract: The present invention is directed to the expansion, preferably TVEMF-expansion, of mammalian blood stem cells in a rotating bioreactor, preferably a TVEMF-bioreactor, to compositions resulting from the expanded and TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions.

Abstract: The present invention is directed to the TVEMF-expansion of mammalian cord blood stem cells in a rotating TVEMF-bioreactor, to compositions resulting from the TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions. Various benefits and advantages to the compositions of the present invention are discussed herein.

Abstract: A method of expanding blood stem cells for the repair of heart tissue and/or function, and compositions resulting from the expansion method in a rotating bioreactor. This invention also relates to a method of TVEMF-expanding blood stem cells for the repair of heart tissue and/or function, and compositions resulting from the TVEMF-expansion in the TVEMF-bioreactor.

Abstract: A method of characterizing a biologically active compound by placing a cell mixture into a rotatable bioreactor to initiate a three-dimensional culture comprising a biological component and at least one cell, controllably expanding the cells in the rotatable bioreactor and testing the biological component to characterize the biologically active compound. The present invention may also preferably comprise exposing the cells to a time varying electromagnetic force.

Abstract: A method of replenishing cells damaged by treatment for cancer comprising removing a sample of peripheral blood containing CD34+ pluripotent adult stem cells from a primate mammal, controllably expanding the cells without differentiation at a rate which produces an expansion factor of at least seven times within seven days while maintaining their three-dimensional geometry and their cell-to-cell support and cell-to-cell geometry, removing any toxic materials from the expanded undifferentiated CD34+ pluripotent adult stem cells, and reintroducing the CD34+ pluripotent adult stem cells into the primate mammal within a time period sufficient to prevent the primate mammal from suffering decreased mobility due to loss of hematopoietic or other cells from a treatment for cancer.

Abstract: Method of researching disease states using TVEMF-expanded cells.

Abstract: Method of treating disease or repairing tissue with compositions comprising mammalian peripheral blood stem cells, preferably CD34+/CD38? cells, and preferably peripheral blood stem cells resulting from TVEMF-expansion.

Abstract: Process for preparing a composition comprising TVEMF-expanded bone marrow stem cells, anti the composition resulting therefrom.

Abstract: Process for preparing a peripheral blood cell composition in a TVEMF-bioreactor.

Abstract: A rotatable time varying electromagnetic force bioreactor comprising a culture chamber having an interior portion that removably receives a cell mixture and an exterior portion with an electrically conductive coil wrapped around the exterior portion, a motor for rotating the culture chamber, and a time varying electromagnetic force source operatively connected to the electrically conductive coil. In use, the time varying electromagnetic force source delivers a time varying electromagnetic force to the interior portion of the culture chamber exposing the mammalian cells therein to a time varying electromagnetic force thereby expanding the mammalian cells.

Abstract: A composition is disclosed with the composition comprising a mixture of natively glycosylated mammalian biological molecules produced by electromagnetically stimulating living mammalian cells.