Abstract: A method for super-resolution fluorescence microscopy includes the following steps: provoking the stochastic activation of fluorescent emitters contained in a sample to be observed, and illuminating the sample with an excitation light beam having a wavelength suitable for inducing fluorescent emission from the activated emitters; and acquiring a sequence of fluorescence images by means of an imaging system comprising a matrix image sensor; measuring arrival delays of fluorescence photons relative to the pulses of the excitation light beam, with a spatial resolution allowing each photon to be associated with a set of pixels of the matrix image sensor. A device and computer program product for the implementation of such a method are also provided.