Abstract: A programmable multiple-point illuminator for an optical microscope (M) comprises a light source (1, 2) and a spatial light modulator (SLM) to modulate a light beam from the light source. The modulated light beam is intended to scan across a sample placed under the microscope objective (21), the sample being provided with fluorophores. The SLM comprises a first acousto-optic deflector (8) and a second acousto-optic deflector (9), the first acousto-optic deflector having a first modulation plane (81) and the second acousto-optic deflector having a second modulation plane (91), said two acousto-optic deflectors being arranged in cascade to provide respective deflection in different directions, whereby the SLM is enabled to scan in two dimensions across the sample. The SLM further comprises a telescope relay (10) to conjugate the first modulation plane with the second modulation plane.

Abstract: A programmable multiple-point illuminator for an optical microscope includes a light source and a spatial light modulator (SLM) to modulate a light beam from the light source. The modulated light beam scans across a sample provided with fluorophores placed under the microscope objective. The SLM includes a first and a second acousto-optic deflectors, said two acousto-optic deflectors being arranged in cascade to provide respective deflection in different directions, whereby the SLM is enabled to scan in two dimensions across the sample. The SLM further includes a telescope relay to conjugate the first modulation plane with the second modulation plane. The illuminator also has an arbitrary waveform generator configured to synthesize holograms, and is arranged to simultaneously inject a first such hologram into the first acousto-optic deflector and a second such hologram into the second acousto-optic deflector, in order to modulate the light beam in response to said holograms.

Abstract: A programmable multiple-point illuminator for an optical microscope (M) including a light source and a spatial light modulator (SLM) to modulate a light beam from the light source. The modulated light beam scans across a sample placed under the microscope objective, the sample being provided with fluorophores. The SLM includes a first acousto-optic deflector and a second acousto-optic deflector, the first acousto-optic deflector having a first modulation plane and the second acousto-optic deflector having a second modulation plane, said two acousto-optic deflectors being arranged in cascade to provide respective deflection in different directions, whereby the SLM is enabled to scan in two dimensions across the sample. The SLM further a telescope relay to conjugate the first modulation plane with the second modulation plane.