Abstract: A convenient, self-contained, disposable device suitable for the rapid identification of microorganisms, having capability for simultaneously carrying out a multiplicity of analytical determinations. The device comprises a disc-shaped arrangement defining a multiplicity of satellite testing chambers each of which is connected to a centrally defined chamber. The central chamber is provided with a movable sleeve or piston arrangement. Liquid sample is received in the central chamber and the sleeve is manipulated to provide samples uniformly and simultaneously to each of the satellite testing chambers without resorting to external means, such as positive or a negative pressurization or centrifugal force. Also disclosed are unique disc-shaped optically transparent support films which are coated on one or both sides with analytical substrates or reagents which are predispensed to the satellite testing chambers.

Abstract: A diagnostic device for measuring biochemical characteristics of microorganisms. A first chamber is provided into which is inoculated a test suspension containing an unknown organism. First passage means selectively provides liquid communication between the first chamber and a second chamber. The second chamber provides for contacting the liquid suspension with a substrate reagent which is located therein. Second passage means connects the second chamber to a third and selectively allows liquid communication therebetween. The third chamber may contain a suitable detection means for identifying the biochemical characteristics of the reacted test suspension when brought into contact therewith.