Abstract: The present invention provides methods for regrowth of conifer embryogenic cells. The methods comprise the steps of (a) contacting cryopreserved conifer embryogenic cells with a liquid transition medium, and (b) culturing the contacted conifer embryogenic cells in a regrowth medium to generate regrowth of the conifer embryogenic cells. Generally, the cryopreserved conifer embryogenic cells are contacted with the liquid transition medium for less than about 24 hours, such as for about one hour.

Abstract: The present invention provides methods for regrowth of conifer embryogenic cells. The methods comprise the steps of (a) contacting cryopreserved conifer embryogenic cells with a liquid transition medium, and (b) culturing the contacted conifer embryogenic cells in a regrowth medium to generate regrowth of the conifer embryogenic cells. Generally, the cryopreserved conifer embryogenic cells are contacted with the liquid transition medium for less than about 24 hours, such as for about one hour.

Abstract: In one aspect, the present invention provides methods for producing Douglas-fir somatic embryos. In one aspect, the methods of the invention comprise the step of culturing Douglas-fir explants in, or on, an initiation medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form embryonal suspensor masses comprising early-stage somatic embryos. In another aspect, the methods of the invention additionally comprise the step of culturing Douglas-fir embryonal suspensor masses in maintenance medium comprising a principal carbohydrate source selected from the group consisting of maltose, glucose, and a combination thereof, to form an established embryonal suspensor mass comprising early-stage somatic embryos.