Patents by Inventor Doris Roth

Doris Roth has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240309331
    Abstract: The present invention relates to a combination of microbes, cell culture systems and microfluidic fluidic systems for use in providing a human Intestine On-Chip with optimal intestinal motility. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells in the presence of bacteria, such as probiotic bacteria, may find use in providing an Intestine-On-Chip for testing intestinal motility function. In some embodiments, an Intestine On-Chip may be used for identifying (testing) therapeutic compounds continuing probiotic microbes or compounds for inducing intestinal motility for use in treating gastrointestinal disorders or diseases related to intestinal function.
    Type: Application
    Filed: February 29, 2024
    Publication date: September 19, 2024
    Inventors: S. Jordan Kerns, Catherine Karalis, Janna Nawroth, Remi Villenave, Jenifer Obrigewitch, Doris Roth, Michael Salmon, Athanasia Apostolou, David Conegliano
  • Publication number: 20210115406
    Abstract: The present invention relates to a combination of microbes, cell culture systems and microfluidic fluidic systems for use in providing a human Intestine On-Chip with optimal intestinal motility. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells in the presence of bacteria, such as probiotic bacteria, may find use in providing an Intestine-On-Chip for testing intestinal motility function. In some embodiments, an Intestine On-Chip may be used for identifying (testing) therapeutic compounds continuing probiotic microbes or compounds for inducing intestinal motility for use in treating gastrointestinal disorders or diseases related to intestinal function.
    Type: Application
    Filed: November 4, 2020
    Publication date: April 22, 2021
    Inventors: S. Jordan Kerns, Catherine Karalis, Janna Nawroth, Remi Villenave, Jenifer Obrigewitch, Doris Roth, Michael Salmon, Athanasia Apostolou, David Conegliano
  • Patent number: 9915650
    Abstract: The present invention relates to an in vitro method for detecting cAMP or cGMP comprising a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to a cAMP quencher, and b) measuring the change in fluorescence. Furthermore, the present invention relates to the use of said method for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP and for screening of a ligand for such a receptor.
    Type: Grant
    Filed: December 13, 2007
    Date of Patent: March 13, 2018
    Assignee: HOFFMANN-LA-ROCHE INC.
    Inventors: Thilo Enderle, Hugues Matile, Doris Roth
  • Publication number: 20140363898
    Abstract: The present invention provides an assay for the identification of beta-sheet aggregated protein ligands using Thiazine Red R.
    Type: Application
    Filed: December 20, 2013
    Publication date: December 11, 2014
    Applicant: HOFFMANN-LA ROCHE INC.
    Inventors: Edilio Borroni, Christian Czech, Arnulf Dorn, Luca Gobbi, Valerie Goetschy-Meyer, Fiona Grueninger, Doris Roth
  • Patent number: 8309310
    Abstract: The present invention relates to a generic method for detecting a kinase or phosphatase activity. The method comprises the following steps: incubating a kinase or phosphatase activity sample with a kinase or phosphatase substrate molecule comprising either a fluorophore having a detectable readout or molecule with an aromatic group which serves as a quencher of the fluorophore, incubating the mixture of step a) with a detection entity comprising either a fluorophore or a molecule with an aromatic group and a binding partner, wherein the substrate molecule and the detection entity are capable of binding to the binding partner and the binding of the substrate molecule and the detection entity to the binding partner lead to an altered readout of the fluorophore, and measuring the readout of the fluorophore in the mixture of step b), wherein an altered readout of the fluorophore compared to a blank is indicative for the presence of a kinase or phosphatase activity in the sample.
    Type: Grant
    Filed: September 1, 2008
    Date of Patent: November 13, 2012
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Thilo Enderle, Doris Roth
  • Patent number: 8206941
    Abstract: The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor and an energy acceptor that are present on the detection molecule and the substrate molecule.
    Type: Grant
    Filed: June 16, 2009
    Date of Patent: June 26, 2012
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Thilo Enderle, Doris Roth
  • Publication number: 20100203554
    Abstract: The present invention relates to a generic method for detecting a kinase or phosphatase activity. The method comprises the following steps: incubating a kinase or phosphatase activity sample with a kinase or phosphatase substrate molecule comprising either a fluorophore having a detectable readout or molecule with an aromatic group which serves as a quencher of the fluorophore, incubating the mixture of step a) with a detection entity comprising either a fluorophore or a molecule with an aromatic group and a binding partner, wherein the substrate molecule and the detection entity are capable of binding to the binding partner and the binding of the substrate molecule and the detection entity to the binding partner lead to an altered readout of the fluorophore, and measuring the readout of the fluorophore in the mixture of step b), wherein an altered readout of the fluorophore compared to a blank is indicative for the presence of a kinase or phosphatase activity in the sample.
    Type: Application
    Filed: September 1, 2008
    Publication date: August 12, 2010
    Inventors: Thilo Enderle, Doris Roth
  • Publication number: 20090253150
    Abstract: The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor and an energy acceptor that are present on the detection molecule and the substrate molecule.
    Type: Application
    Filed: June 16, 2009
    Publication date: October 8, 2009
    Inventors: Thilo Enderle, Doris Roth
  • Publication number: 20080153123
    Abstract: The present invention relates to an in vitro method for detecting cAMP or cGMP comprising a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to a cAMP quencher, and b) measuring the change in fluorescence. Furthermore, the present invention relates to the use of said method for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP and for screening of a ligand for such a receptor.
    Type: Application
    Filed: December 13, 2007
    Publication date: June 26, 2008
    Inventors: Thilo Enderle, Hugues Matile, Doris Roth
  • Publication number: 20070009978
    Abstract: The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor and an energy acceptor that are present on the detection molecule and the substrate molecule.
    Type: Application
    Filed: July 20, 2006
    Publication date: January 11, 2007
    Inventors: Thilo Enderle, Doris Roth