Abstract: Disclosed embodiments include a tray configured for holding various sterilization targets. The tray may include a perforated floor member connected to a plurality of vertically oriented walls, which may also be perforated. Together, the floor member and walls define an internal cavity that includes at least one wire support for removably holding at least one sterilization target, such as a medical instrument and/or implant or components thereof, within the internal cavity. Each wire support may include two end inserts and a middle portion, the two inserts secured via connection means to one or more walls of the tray. The connection means may comprise mounting fixtures that further include a plurality of receiving slots or holes each configured to receive an insert. The wire supports may increase sterilant flow and circulation, thereby improving sterilization of instruments and/or implants held within the tray disclosed herein.