Abstract: A biological indicator (100) for use in a liquid-chemical decontamination system is disclosed. The biological indicator may include a housing (102) and a cap (106) coupled to the housing. The cap may comprise a liquid chamber and at least two ports. One of the ports may connect to a liquid passage in the cap such that liquids such as liquid-chemical decontaminants may be introduced and removed from the biological indicator via this port.

Abstract: A class 5 chemical indicator color may be imaged such that a color of the indicator may be determined and compared to a color-value threshold indicative of an efficacious sterilization procedure. Color changes of the indicator during a sterilization procedure are typically not uniform. As such, colors on the indicator may be averaged and then compared to the color-value threshold to determine if the procedure has been efficacious or if additional exposure to a sterilant may be desired. Color values and color-value thresholds may be based on, e.g., the L*a*b* color model or a grayscale color model.