Patents by Inventor Doug WHITMAN
Doug WHITMAN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230068047Abstract: Provided herein are methods and systems for detecting the presence or absence of multiple target nucleic acids in partitions of a digital amplification assay. In one embodiment of the method, different probes labeled with the same signal-generating label are distinguishable from each other as a result of having different melting temperatures in the presence of their cognate target nucleic acids. Following amplification of the target nucleic acids in a digital assay, signals are measured at three or more different temperatures and at least two relational values between signals measured at the three or more different temperatures are calculated and plotted against each other to classify partition subsets according to their target nucleic acid occupancy.Type: ApplicationFiled: August 17, 2022Publication date: March 2, 2023Applicant: LUMINEX CORPORATIONInventors: Jenni BERNIER, Doug WHITMAN
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Publication number: 20220282307Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.Type: ApplicationFiled: May 25, 2022Publication date: September 8, 2022Applicant: LUMINEX CORPORATIONInventor: Doug WHITMAN
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Patent number: 11371080Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.Type: GrantFiled: November 21, 2019Date of Patent: June 28, 2022Assignee: LUMINEX CORPORATIONInventor: Doug Whitman
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Patent number: 11371082Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5? of the target-specific primer region; and a second stem forming region 3? of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.Type: GrantFiled: May 8, 2020Date of Patent: June 28, 2022Assignee: LUMINEX CORPORATIONInventors: Martin Kochanczyk, Doug Whitman
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Publication number: 20220154257Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.Type: ApplicationFiled: January 31, 2022Publication date: May 19, 2022Applicant: LUMINEX CORPORATIONInventors: Doug WHITMAN, Nicolas ARAB, Chuck COLLINS
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Patent number: 11261481Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.Type: GrantFiled: February 28, 2020Date of Patent: March 1, 2022Assignee: LUMINEX CORPORATIONInventors: Doug Whitman, Nicolas Arab, Chuck Collins
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Publication number: 20210310056Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In one embodiment, a sample is contacted with a primer and a quencher-probe complementary to a target nucleic acid. The quencher-probe is complementary to an anti-probe that comprises a reporter and is attached to a solid support. Thus, hybridized probe is cleaved with a nucleic acid polymerase having exonuclease activity to release the quencher from the probe. The presence of the target nucleic acid is then detected and/or optionally quantified by detecting an increase in signal from the fluorescent reporter on the solid support.Type: ApplicationFiled: June 18, 2021Publication date: October 7, 2021Applicant: LUMINEX CORPORATIONInventors: Doug WHITMAN, Brian SCHRADER
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Publication number: 20210189469Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.Type: ApplicationFiled: March 9, 2021Publication date: June 24, 2021Applicant: LUMINEX CORPORATIONInventors: Scott C. JOHNSON, Nicolas ARAB, Doug WHITMAN
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Patent number: 10975419Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.Type: GrantFiled: April 25, 2018Date of Patent: April 13, 2021Assignee: LUMINEX CORPORATIONInventors: Scott C. Johnson, Nicolas Arab, Doug Whitman
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Patent number: 10851336Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.Type: GrantFiled: November 28, 2018Date of Patent: December 1, 2020Assignee: LUMINEX CORPORATIONInventors: Doug Whitman, James Heisler, Colin Bozarth, Germaine Siew, Charles Collins, Eric Smith
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Publication number: 20200362396Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.Type: ApplicationFiled: August 3, 2020Publication date: November 19, 2020Applicant: LUMINEX CORPORATIONInventors: Scott C. JOHNSON, Nicolas ARAB, Doug WHITMAN
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Patent number: 10752939Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.Type: GrantFiled: July 21, 2017Date of Patent: August 25, 2020Assignee: LUMINEX CORPORATIONInventors: Scott C. Johnson, Nicolas Arab, Doug Whitman
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Publication number: 20200263240Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5? of the target-specific primer region; and a second stem forming region 3? of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.Type: ApplicationFiled: May 8, 2020Publication date: August 20, 2020Applicant: LUMINEX CORPORATIONInventors: Martin KOCHANCZYK, Doug WHITMAN
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Publication number: 20200199658Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.Type: ApplicationFiled: February 28, 2020Publication date: June 25, 2020Applicant: LUMINEX CORPORATIONInventors: Doug WHITMAN, Nicolas ARAB, Chuck COLLINS
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Patent number: 10683535Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5? of the target-specific primer region; and a second stem forming region 3? of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.Type: GrantFiled: June 6, 2018Date of Patent: June 16, 2020Assignee: LUMINEX CORPORATIONInventors: Martin Kochanczyk, Doug Whitman
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Publication number: 20200172964Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.Type: ApplicationFiled: November 21, 2019Publication date: June 4, 2020Applicant: LUMINEX CORPORATIONInventor: Doug WHITMAN
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Patent number: 10612074Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.Type: GrantFiled: April 28, 2017Date of Patent: April 7, 2020Assignee: LUMINEX CORPORATIONInventors: Doug Whitman, Nicolas Arab, Chuck Collins
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Publication number: 20190093061Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.Type: ApplicationFiled: November 28, 2018Publication date: March 28, 2019Applicant: LUMINEX CORPORATIONInventors: Doug WHITMAN, James HEISLER, Colin BOZARTH, Germaine SIEW, Charles COLLINS, Eric SMITH
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Patent number: 10179899Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.Type: GrantFiled: June 5, 2015Date of Patent: January 15, 2019Assignee: LUMINEX CORPORATIONInventors: Doug Whitman, James Heisler, Colin Bozarth, Germaine Siew, Charles Collins, Eric Smith
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Publication number: 20180355415Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5? of the target-specific primer region; and a second stem forming region 3? of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.Type: ApplicationFiled: June 6, 2018Publication date: December 13, 2018Applicant: LUMINEX CORPORATIONInventors: Martin KOCHANCZYK, Doug WHITMAN