Abstract: Disclosed are methods of synthesizing a polynucleotide complementary to a target polynucleotide include steps of subjecting a non-thermophilic cell comprising a thermostable polymerase to a temperature effective to disrupt the cell to form a reaction mixture, wherein the reaction mixture comprises the target polynucleotide and one or more primers that hybridize to a sequence of the target polynucleotide or to a sequence flanking the polynucleotide and incubating the reaction mixture under conditions whereby the polynucleotide is synthesized. Also disclosed are cell libraries and kits in accordance with the invention.

Abstract: An exercise device employing side-by-side pivotally supported moving surfaces. In one particular example, an exercise device employs a first belt deployed about a front roller and a rear roller and an adjacent second belt deployed about a front roller and a rear roller. The rear of the belts in the area of the rear rollers are pivotally secured and the front of the belts in the area of the front roller are adapted to reciprocate in an up and down motion during use. In some implementations, the moving surfaces include an interconnection structure such that a generally downward movement of one surface is coordinated with a generally upward movement of the other surface. In other implementations, the moving surfaces are operably associated with one or more resistance elements that effect the amount of force required to pivot or actuate the moving surfaces.

Abstract: The various embodiments of the present invention generally provide a control system and a process for an exercise apparatus configurable into a combined treadmill and stepper mode. The apparatus may also be configured into stepper only and treadmill only modes. The apparatus generally includes a master control unit, a first sensor, in communication with the master control unit, which generates a first signal indicative of an effective tread speed for the apparatus, and a resistive element that includes at least one resistance level. Using the first signal, the resistance level, and empirical information, the amount of energy expended by a user of the apparatus may be calculated and the operation of the apparatus controlled. Various sensors, actuators and information, such as that obtained from various data structures, may be utilized in performing calculations and controlling the features, functions and operation of the apparatus.

Abstract: An exercise device employing side-by-side pivotally supported moving surfaces. In one particular example, an exercise device employs a first belt deployed about a front roller and a rear roller and an adjacent second belt deployed about a front roller and a rear roller. The rear of the belts in the area of the rear rollers are pivotally secured and the front of the belts in the area of the front roller are adapted to reciprocate in an up and down motion during use. In some implementations, the moving surfaces include an interconnection structure such that a generally downward movement of one surface is coordinated with a generally upward movement of the other surface. In other implementations, the moving surfaces are operably associated with one or more resistance elements that effect the amount of force required to pivot or actuate the moving surfaces.

Abstract: A dumbbell is described including a handle having a grip and at least one end, an inner plate mounted on the handle adjacent the grip, in a fixed rotational orientation, a support plate rotationally mounted on the handle adjacent the inner plate, at least one collar rotationally mounted on the handle adjacent the support plate, and rotationally fixed with the support plate, a selector knob rotationally mounted on the handle adjacent the at least one collar, and rotationally fixed with the collar, a weight plate removably mounted on the handle adjacent the at least one collar, and a means for selectively securing the support plate to the inner plate to resist the rotation of the support plate, collar and selector knob with respect to the inner plate and handle.

Abstract: The invention involves a base handle bar configuration having a stem and a base gripping portion connected with the stem. A variety of combinations of stub grips, spanning bars, arcuate bars, cross bars, prong grips, and the like are connected with the base gripping portion. The handle bar may be connected with any type of exercise device and is particularly suited for connection with an exercise bicycle. The various handlebar configurations provide a user with numerous gripping alternatives during use of the exercise device.